Abstract
Lectins and polyclonal antiserum that bind specifically and reproducibly to the overall surface of Paratrichodorus anemones were identified and bound to monodisperse superparamagnetic particles (Dynabeads) to assess their efficiency as probes for capturing target nematodes from test suspensions. In recovery experiments, while both types of probe isolated nematodes, antibody-coated beads recovered them more efficiently than beads coated with lectins. When immunomagnetic capture was used to isolate P. anemones from mixtures of naturally occurring populations of nematodes extracted in bulk, from soil samples, 80% of the target nematodes were recovered.
This is a preview of subscription content, log in via an institution to check access.
References
Alphey, T. J. W., & Taylor, C. E. (1986). European atlas of the longidoridae and trichodoridae. Dundee: Scottish Crop Research Institute.
Boag, B. (1981). Observations on the population dynamics and vertical distribution of trichodorid nematodes in a Scottish forest nursery. Annals of Applied Biology, 98, 463–469.
Boutsika, K., Phillips, M. S., MacFarlane, S. A., Brown, D. J. F., Holeva, R. C., & Blok, V. C. (2004). Molecular diagnostics of some trichodorid nematodes and associated Tobacco rattle virus. Plant Pathology, 53, 110–116.
Brooks, S. A., Leathem, A. J. C., & Schumacher, U. (1996). Lectin histochemistry. Oxford: BIOS Scientific.
Brown, D. J. F., & Boag, B. (1988). An examination of methods used to extract virus-vector nematodes (Nematoda: Longidoridae and Trichodoridae) from soil samples. Nematologia Mediterranea, 16, 93–99.
Chen, Q., Brown, D. J. F., Curtis, R. H., & Jones, J. T. (2003). Development of the magnetic capture system for recovery of Xiphinema americanum. Annals of Applied Biology, 143, 283–289.
Chen, Q., Robertson, L., Jones, J. T., Blok, V. C., Phillips, M. S., & Brown, D. J. F. (2001). Capture of nematodes using antiserum and lectin-coated magnetised beads. Nematology, 3, 593–601.
Decraemer, W. (1995). The family Trichodoridae: Stubby root and virus vector nematodes. Dordrecht: Kluwer.
Decraemer, W., & Baujard, P. (1998). A polytomous key for the identification of species of the family Trichodoridae Thorne, 1935 (Nematoda: Triplonchida). Fundamental and Applied Nematology, 21, 37–62.
Duncan, L. H., Robertson, L., Robertson, W. M., & Kusel, J. R. (1997). Isolation and characterization of secretions from the plant-parasitic nematode Globodera pallida. Parasitology, 115, 429–438.
Galfré, G., & Milstein, C. (1981). Preparation of monoclonal antibodies: Strategies and procedures. Methods in Enzymology, 73, 1–46.
Holeva, R., Phillips, M. S., Neilson, R., Brown, D. J. F., Young, V., Boutsika, K., et al. (2006). Real-time PCR detection and quantification of vector trichodorid nematodes and Tobacco rattle virus. Molecular and Cellular Probes, 20, 203–211.
Lund, A., Hellenmann, A. L., & Vartdal, F. (1988). Rapid isolation of K88+ Escherichia coli by using immunomagnetic particles. Journal of Clinical Microbiology, 26, 2572–2575.
MacFarlane, S. A., Neilson, R., & Brown, D. J. F. (2002). Nematodes. Advances in Botanical Research, 36, 169–198.
Morgan, J. A. W., Winstanley, C., Pickup, R. W., & Saunders, J. R. (1991). Rapid immunocapture of Pseudomonas putida cells from lake water by using bacterial flagella. Applied and Environmental Microbiology, 57, 503–509.
Powers, T. O. (2004). Nematode molecular diagnostics: From bands to barcodes. Annual Review of Phytopathology, 42, 367–383.
Stryer, L. (1995). Biochemistry. New York: Freeman.
Subbotin, S. A., Peng, D. L., & Moens, M. (2001). A rapid method for the identification of the soybean cyst nematode Heterodera glycines using duplex PCR. Nematology, 3, 365–371.
Taylor, C. E., & Brown, D. J. F. (1997). Nematode vectors of plant viruses. Wallingford: CABI International.
Van Damme, E. J. M., Peumans, W. J., Pusztai, A., & Bardocz, S. (1998). Handbook of plant lectins: Properties and biomedical applications. Chichester: Wiley.
Acknowledgement
RCH was funded by the British Potato Council. Research at the Scottish Crop Research Institute is grant-aided by the Scottish Executive Environment and Rural Affairs Department.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Holeva, R.C., Brown, D.J.F., Curtis, R.H.C. et al. Selective recovery of the virus-vector trichodorid nematode Paratrichodorus anemones from soil samples by immunomagnetic capture. Eur J Plant Pathol 120, 85–89 (2008). https://doi.org/10.1007/s10658-007-9192-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10658-007-9192-y