European Journal of Plant Pathology

, Volume 118, Issue 1, pp 43–57 | Cite as

Biological control of Botrytis cinerea by selected grapevine-associated bacteria and stimulation of chitinase and β-1,3 glucanase activities under field conditions

  • Maryline Magnin-Robert
  • Patricia Trotel-Aziz
  • Daniel Quantinet
  • Sylvie Biagianti
  • Aziz Aziz
Full Research Paper


In this study, the biocontrol ability of seven grapevine-associated bacteria, previously reported as efficient against Botrytis cinerea under in vitro conditions, was evaluated in two vineyard orchards with the susceptible cv. Chardonnay during four consecutive years (2002–2005). It was shown that the severity of disease on grapevine leaves and berries was reduced to different levels, depending on the bacterial strain and inoculation method. Drenching the plant soil with these bacteria revealed a systemic resistance to B. cinerea, even without renewal of treatment. Accordingly, this resistance was associated with a stimulation of some plant defense responses such as chitinase and β-1,3-glucanase activities in both leaves and berries. In leaves, chitinase activity increased before veraison (end-July) while β-1,3-glucanase reached its maximum activity at ripening (September). Reverse patterns were observed in berries, with β-1,3-glucanase peaking at full veraison (end-August) and chitinase at a later development stage. Highest activities were observed with Acinetobacter lwoffii PTA-113 and Pseudomonas fluorescens PTA-CT2 in leaves, and with A. lwoffii PTA-113 and Pantoea agglomerans PTA-AF1 in berries. These results have demonstrated an induced protection of grapevine against B. cinerea by selected bacteria under field conditions, and suggest that induced resistance could be related to a stimulation of plant defense reactions in a successive manner.


Biocontrol Grey mould Induced resistance Rhizobacteria Vitis vinifera



We thank A. Conreux for her technical assistance and the GDV members for their help with disease evaluation in vineyards. We also gratefully acknowledge financial support from Europôl’Agro (Reims-France).


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Copyright information

© KNPV 2007

Authors and Affiliations

  • Maryline Magnin-Robert
    • 1
    • 2
  • Patricia Trotel-Aziz
    • 1
    • 2
  • Daniel Quantinet
    • 3
  • Sylvie Biagianti
    • 1
  • Aziz Aziz
    • 1
    • 2
  1. 1.Laboratoire d’Eco-Toxicologie, URVVC—EA 2069Université de ReimsReims cedex 2France
  2. 2.Laboratoire de Plantes, Pesticides et Développement Durable, URVVC—EA 2069Université de ReimsReims cedex 2France
  3. 3.GDV de la MarneInstitut Technique de ChampagneEpernay cedexFrance

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