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Biological control of Botrytis cinerea by selected grapevine-associated bacteria and stimulation of chitinase and β-1,3 glucanase activities under field conditions

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Abstract

In this study, the biocontrol ability of seven grapevine-associated bacteria, previously reported as efficient against Botrytis cinerea under in vitro conditions, was evaluated in two vineyard orchards with the susceptible cv. Chardonnay during four consecutive years (2002–2005). It was shown that the severity of disease on grapevine leaves and berries was reduced to different levels, depending on the bacterial strain and inoculation method. Drenching the plant soil with these bacteria revealed a systemic resistance to B. cinerea, even without renewal of treatment. Accordingly, this resistance was associated with a stimulation of some plant defense responses such as chitinase and β-1,3-glucanase activities in both leaves and berries. In leaves, chitinase activity increased before veraison (end-July) while β-1,3-glucanase reached its maximum activity at ripening (September). Reverse patterns were observed in berries, with β-1,3-glucanase peaking at full veraison (end-August) and chitinase at a later development stage. Highest activities were observed with Acinetobacter lwoffii PTA-113 and Pseudomonas fluorescens PTA-CT2 in leaves, and with A. lwoffii PTA-113 and Pantoea agglomerans PTA-AF1 in berries. These results have demonstrated an induced protection of grapevine against B. cinerea by selected bacteria under field conditions, and suggest that induced resistance could be related to a stimulation of plant defense reactions in a successive manner.

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Acknowledgements

We thank A. Conreux for her technical assistance and the GDV members for their help with disease evaluation in vineyards. We also gratefully acknowledge financial support from Europôl’Agro (Reims-France).

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Authors and Affiliations

  1. Laboratoire d’Eco-Toxicologie, URVVC—EA 2069, Université de Reims, B.P. 1039, 51687, Reims cedex 2, France

    Maryline Magnin-Robert, Patricia Trotel-Aziz, Sylvie Biagianti & Aziz Aziz

  2. Laboratoire de Plantes, Pesticides et Développement Durable, URVVC—EA 2069, Université de Reims, B.P. 1039, 51687, Reims cedex 2, France

    Maryline Magnin-Robert, Patricia Trotel-Aziz & Aziz Aziz

  3. GDV de la Marne, Institut Technique de Champagne, 17 rue Jean Chandon-Moët, 51202, Epernay cedex, France

    Daniel Quantinet

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  1. Maryline Magnin-Robert
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  2. Patricia Trotel-Aziz
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  3. Daniel Quantinet
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  4. Sylvie Biagianti
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  5. Aziz Aziz
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Correspondence to Aziz Aziz.

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Magnin-Robert, M., Trotel-Aziz, P., Quantinet, D. et al. Biological control of Botrytis cinerea by selected grapevine-associated bacteria and stimulation of chitinase and β-1,3 glucanase activities under field conditions. Eur J Plant Pathol 118, 43–57 (2007). https://doi.org/10.1007/s10658-007-9111-2

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