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Molecular characterisation of vegetative compatibility groups in Fusarium oxysporum f. sp. radicis-lycopersici and f. sp. lycopersici by random amplification of polymorphic DNA and microsatellite-primed PCR

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Abstract

Random amplification of polymorphic DNA (RAPD-PCR) analysis was conducted on 48 isolates of Fusarium oxysporum f. sp. radicis-lycopersici (F.o.r.l.) from different geographic regions, representing all known vegetative compatibility groups (VCGs) except VCG 0097 and VCG 0099 and on eight isolates of F.oxysporum f. sp. lycopersici (F.o.l.), representing VCGs 0030, 0031, 0032 and 0033. Upon UPGMA (unweighted pair-group method with arithmetic averages) analysis of 86 RAPD-PCR markers generated by 16 informative primers and 44 markers obtained with eight microsatellite primers, a close relatedness was evident for F.o.r.l. isolates in VCGs 0090, 0092, 0096, and, to a lesser extent, for those in VCG 0093. Representatives of VCG 0091 formed a distinct group, while F.o.r.l. isolates in VCGs 0094 and 0098 were not distinguishable by the tested markers, most of which were also shared by F.o.l. isolates belonging to VCGs 0031 and 0033. F.o.l. isolates in VCGs 0030 and 0032 shared most of the molecular markers. The correlation between RAPD-PCR and microsatellite genetic distance was highly significant (R 2 = 0.77; P by Mantel test < 0.001). The molecular variability observed in both formae speciales is discussed in relation to the development of F.o.r.l.- and F.o.l.-specific diagnostic tools.

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Author notes

  1. Pietro Di Primo

    Present address: The Biological Division, AgroGreen, Minrav Infrastructures LTD, Kiriat Moriah, P.O.B. 29229, 91291, Jerusalem, Israel

Authors and Affiliations

  1. Dipartimento di Protezione delle Piante – Center for Biotechnology Development and Biodiversity Research, University of Sassari, Via Enrico De Nicola 9, I-07100, Sassari, Italy

    Virgilio Balmas, Barbara Scherm, Angela Marcello & Quirico Migheli

  2. Dipartimento di Agrochimica ed Agrobiologia, Università “Mediterranea” di Reggio Calabria, Località Feo di Vito, I-89060, Reggio Calabria, Italy

    Pietro Di Primo

  3. Dipartimento di Scienze Agronomiche e Genetica Vegetale Agraria, University of Sassari, Via Enrico De Nicola 9, I-07100, Sassari, Italy

    Domenico Rau

Authors
  1. Virgilio Balmas
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  2. Barbara Scherm
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  3. Pietro Di Primo
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  4. Domenico Rau
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  5. Angela Marcello
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  6. Quirico Migheli
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Correspondence to Quirico Migheli.

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Balmas, V., Scherm, B., Di Primo, P. et al. Molecular characterisation of vegetative compatibility groups in Fusarium oxysporum f. sp. radicis-lycopersici and f. sp. lycopersici by random amplification of polymorphic DNA and microsatellite-primed PCR. Eur J Plant Pathol 111, 1–8 (2005). https://doi.org/10.1007/s10658-004-1602-9

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  • DOI: https://doi.org/10.1007/s10658-004-1602-9

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