Increase of multi-metal tolerance of three leguminous plants by arbuscular mycorrhizal fungi colonization
- 317 Downloads
A greenhouse pot experiment was conducted to investigate the effects of the colonization of arbuscular mycorrhizal fungus (AMF) Glomus mosseae on the growth and metal uptake of three leguminous plants (Sesbania rostrata, Sesbania cannabina, Medicago sativa) grown in multi-metal contaminated soil. AMF colonization increased the growth of the legumes, indicating that AMF colonization increased the plant’s resistance to heavy metals. It also significantly stimulated the formation of root nodules and increased the N and P uptake of all of the tested leguminous plants, which might be one of the tolerance mechanisms conferred by AMF. Compared with the control, colonization by G. mosseae decreased the concentration of metals, such as Cu, in the shoots of the three legumes, indicating that the decreased heavy metals uptake and growth dilution were induced by AMF treatment, thereby reducing the heavy metal toxicity to the plants. The root/shoot ratios of Cu in the three legumes and Zn in M. sativa were significantly increased (P < 0.05) with AMF colonization, indicating that heavy metals were immobilized by the mycorrhiza and the heavy metal translocations to the shoot were decreased.
KeywordsSesbania Medicago sativa Glomus mosseae A. caulinodans Heavy metals
This work was financially supported by the Research Grants Council (HKBU2181/03M), Hong Kong, China, the Ministry of Science and Technology (2002CB410808), China and the Young Scholars Fund of Beijing University of Chemical Technology, China (QN0603).
- Andrade, M. R., Farina, M., & Amado Filho, G. M. (2002). Role of Padina gymnospora (Dictyotales, Phaeophyceae) call walls in cadmium accumulation. Phycologia, 41, 39–48.Google Scholar
- Ibekwe, A. M., Angle, J. S., Chaney, R. L., & Van-Berkum, P. (1995). Sewage sludge and heavy metal effects on nodulation and nitrogen fixation of legumes. Journal of Environmental Quality, 24,1199–1204.Google Scholar
- Kotrba, P., Doleckova, L., Lorenzo, V., & Rumi, T. (1999). Enhanced bioaccumulation of heavy metal ions by bacterial cells due to surface display of short metal binding peptides. Applied and Environmental Microbiology, 65, 1092–1098.Google Scholar
- Payne, R. W., (Ed.), (2002). The guide to GenStat Release 6.1—Part 1: Syntax and data management. GenStat Committee. Hemel Hempstead, UK: VSN International.Google Scholar
- Smith, S. E., & Read, D. J. (1997). Mycorrhizal Simbiosis. London: Academic Press.Google Scholar
- Tiemann, K. J., Gardea-Torresdey, J. L., Gamez, G., Dokken, K., Sias, S., Renner, M. W., Furenlid, L. R. (1999). Use of X-ray absorption apectroscopy and esterification to investigate Cr(III) and Ni(II) ligands in Alfalfa biomass. Environmental Science & Technology, 33, 150–154.CrossRefGoogle Scholar
- Toro, M., Azcón, R., & Barea, J. M. (1998). The use of isotopic dilution techniques to evaluate the interactive effects of Rhizobium genotype, mycorrhiza fungi, phosphate-solubilizing Rhizobacteria and rock phosphate on nitrogen and phosphorus acquisition by Medicago sativa. New Phytologist, 138, 265–273.CrossRefGoogle Scholar