Abstract
Methanotrophs are of great significance for the abatement of methane emission from anoxic environments. Antibiotics are ubiquitous in the environment and can affect microbial activity and community density and composition. However, information about the effect of antibiotics on methanotrophs is still lacking. The current study explored the influences of sulfonamides and tetracyclines on methane oxidation potential (MOP) and methanotrophic density and community structure in freshwater sediment microcosms. The addition of both sulfanilamide (SA) and oxytetracycline (OTC) could increase MOP and particulate methane monooxygenase subunit A (pmoA) gene density but decrease the number of pmoA transcripts. Both SA and OTC could also have impacts on sediment methanotrophic community structure. The antibiotic effects on MOP and methanotrophs were found to depend on the dosage and type of antibiotics. This work could provide some new insights towards the links between methane oxidation and antibiotics.
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References
Aydin S, Ince B, Ince O (2015) Application of real-time PCR to determination of combined effect of antibiotics on bacteria, methanogenic archaea, archaea in anaerobic sequencing batch reactors. Water Res 76:88–98
Bastviken D, Cole J, Pace M, de Bogert M (2008) Fates of methane from different lake habitats, Connecting whole-lake budgets and CH4 emissions. J Geophys Res-Biogeosci 113:G02024
Chen SL, Chen JF, Chang S, Yi H, Huang DW, Xie SG, Guo QW (2018) Aerobic and anaerobic methanotrophic communities in urban landscape wetland. Appl Microbiol Biotechnol 102:433–445
Chessa L, Pusino A, Garau G, Mangia NP, Pinna MV (2016) Soil microbial response to tetracycline in two different soils amended with cow manure. Environ Sci Pollut Res 23:5807–5817
Clarke KR, Warwick RM (2001) Change in marine communities, an approach to statistical analysis and interpretation, 2nd ed. Plymouth, Plymouth Marine Laboratory, [PRIMER-E]
Coban H, Ertekin E, Ince O, Turker G, Akyol C, Ince B (2016) Degradation of oxytetracycline and its impacts on biogas-producing microbial community structure. Bioprocess Biosyst Eng 39:1051–1060
Ding C, He JZ (2010) Effect of antibiotics in the environment on microbial populations. Appl Microbiol Biotechnol 87:925–941
Fang H, Han LX, Cui YL, Xue YF, Cai L, Yu YL (2016) Changes in soil microbial community structure and function associated with degradation and resistance of carbendazim and chlortetracycline during repeated treatments. Sci Total Environ 572:1203–1212
Fang H, Han YL, Yin YM, Jin XX, Wang SY, Tang FF, Cai L, Yu YL (2014) Microbial response to repeated treatments of manure containing sulfadiazine and chlortetracycline in soil. J Environ Sci Health Part B-Pestic Contam Agric Wastes 49:609–615
Gothwal R, Shashidhar T (2015) Antibiotic pollution in the environment: a review. Clean-Soil Air Water 43:479–489
Hanson RS, Hanson TE (1996) Methanotrophic bacteria. Microbiol Rev 60:439
Jiang BC, Li A, Cui D, Cai R, Ma F, Wang YN (2014a) Biodegradation and metabolic pathway of sulfamethoxazole by Pseudomonas psychrophila HA-4, a newly isolated cold-adapted sulfamethoxazole-degrading bacterium. Appl Microbiol Biotechnol 98:4671–468
Jiang YH, Li MX, Guo CS, An D, Xu J, Zhang Y, Xi BD (2014b) Distribution and ecological risk of antibiotics in a typical effluent–receiving river (Wangyang River) in north China. Chemosphere 112:267–274
Li S, Shi WZ, Liu W, Li HM, Zhang W, Hu JR, Ke YC, Sun WL, Ni JR (2018) A duodecennial national synthesis of antibiotics in China’s major rivers and seas (2005–2016). Sci Total Environ 615:906–917
Lin H, Jin DF, Freitag TE, Sun WC, Yu QG, Fu JR, Ma JW (2016) A compositional shift in the soil microbiome induced by tetracycline, sulfamonomethoxine and ciprofloxacin entering a plant-soil system. Environ Pollut 212:440–448
Liu F, Wu JS, Ying GG, Luo ZX, Feng H (2012) Changes in functional diversity of soil microbial community with addition of antibiotics sulfamethoxazole and chlortetracycline. Appl Microbiol Biotechnol 95:1615–1623
Liu B, Li YX, Zhang XL, Wang J, Gao M (2014) Combined effects of chlortetracycline and dissolved organic matter extracted from pig manure on the functional diversity of soil microbial community. Soil Biol Biochem 74:148–155
Liu Y, Zhang JX, Zhao L, Li YZ, Yang YY, Xie SG (2015a) Aerobic and nitrite-dependent methane-oxidizing microorganisms in sediments of freshwater lakes on the Yunnan Plateau. Appl Microbiol Biotechnol 99:2371–2381
Liu B, Li YX, Zhang XL, Wang J, Gao M (2015b) Effects of chlortetracycline on soil microbial communities, comparisons of enzyme activities to the functional diversity via Biolog EcoPlates (TM). Eur J Soil Biol 68:69–76
Liu AJ, Cao HS, Yang Y, Ma XX, Liu X (2016) Combinational effects of sulfomethoxazole and copper on soil microbial community and function. Environ Sci Pollut Res 23:4235–4241
Liu H, Yang YK, Sun HF, Zhao L, Liu Y (2018) Effect of tetracycline on microbial community structure associated with enhanced biological N&P removal in sequencing batch reactor. Bioresour Technol 256:414–420
Lu XQ, Zhen GY, Liu Y, Hojo T, Estrada AL, Li YY (2014) Long-term effect of the antibiotic cefalexin on methane production during waste activated sludge anaerobic digestion. Bioresour Technol 169:644–651
Ma TT, Pan X, Chen LK, Liu WX, Christie P, Luo YM, Wu LH (2016) Effects of different concentrations and application frequencies of oxytetracycline on soil enzyme activities and microbial community diversity. Eur J Soil Biol 76:53–60
Molaei A, Lakzian A, Datta R, Haghnia G, Astaraei A, Rasouli-Sadaghiani M, Ceccherini MT (2017) Impact of chlortetracycline and sulfapyridine antibiotics on soil enzyme activities. Int Agrophys 31:499–505
Op den Camp HJM, Islam T, Stott MB, Harhangi HR, Hynes A, Schouten S, Jetten MSM, Birkeland NK, Pol A, Dunfield PF (2009) Environmental, genomic and taxonomic perspectives on methanotrophic Verrucomicrobia. Environ Microbiol Rep 1:293–306
Park J, Yamashita N, Wu GX, Tanaka H (2017) Removal of pharmaceuticals and personal care products by ammonia oxidizing bacteria acclimated in a membrane bioreactor: contributions of cometabolism and endogenous respiration. Sci Total Environ 605:18–25
Pinna MV, Castaldi P, Deiana P, Pusino A, Garau G (2012) Sorption behavior of sulfamethazine on unamended and manure-amended soils and short-term impact on soil microbial community. Ecotox Environ Safe 84:234–242
Rahalkar M, Deutzmann J, Schink B, Bussmann I (2009) Abundance and activity of methanotrophic bacteria in littoral and profundal sediments of Lake Constance (Germany). Appl Environ Microbiol 75:119–126
Reichel R, Michelini L, Ghisi R, Thiele-Bruhn S (2015) Soil bacterial community response to sulfadiazine in the soil-root zone. J Plant Nutr Soil Sci 178:499–506
Shrestha M, Shrestha PM, Frenzel P, Conrad R (2010) Effect of nitrogen fertilization on methane oxidation, abundance, community structure, and gene expression of methanotrophs in the rice rhizosphere. ISME J 4:1545–1556
Stone JJ, Dreis EK, Lupo CD, Clay SA (2011) Land application of tylosin and chlortetracycline swine manure, Impacts to soil nutrients and soil microbial community structure. J Environ Sci Health Part B-Pestic Contam Agric Wastes 46:752–762
Thiele-Bruhn S, Beck IC (2005) Effects of sulfonamide and tetracycline antibiotics on soil microbial activity and microbial biomass. Chemosphere 59:457–465
Xi XP, Wang M, Chen YS, Yu S, Hong YW, Ma J, Wu Q, Lin QY, Xu XR (2015) Adaption of the microbial community to continuous exposures of multiple residual antibiotics in sediments from a salt-water aquacultural farm. J Hazard Mater 290:96–105
Xiong WG, Sun YX, Ding XY, Wang MZ, Zeng ZL (2015) Selective pressure of antibiotics on ARGs and bacterial communities in manure-polluted freshwater-sediment microcosms. Front Microbiol 6:194
Xiong YH, Harb M, Hong PY (2017) Performance and microbial community variations of anaerobic digesters under increasing tetracycline concentrations. Appl Microbiol Biotechnol 101:5505–5517
Yang H, Xie P, Ni LY, Fower RJ (2011) Underestimation of CH4 emission from freshwater lakes in China. Environ Sci Technol 45:4203–4204
Yang YY, Zhang JX, Zhao Q, Zhou QH, Li NN, Wang YL, Xie SG, Liu Y (2016) Sediment ammonia-oxidizing microorganisms in two plateau freshwater lakes at different trophic states. Microb Ecol 71:257–265
Yang YY, Li NN, Wang W, Li BX, Xie SG, Liu Y (2017) Vertical profiles of sediment methanogenic potential and communities in two plateau freshwater lakes. Biogeosciences 14:341–351
Yang YY, Chen JF, Li BQ, Liu Y, Xie SG (2018) Anaerobic methane oxidation potential and bacteria in freshwater lakes: Seasonal changes and the influence of trophic status. Syst Appl Microbiol 41:650–657
Zhang YY, Geng JJ, Ma HJ, Ren HQ, Xu K, Ding LL (2016) Characterization of microbial community and antibiotic resistance genes in activated sludge under tetracycline and sulfamethoxazole selection pressure. Sci Total Environ 571:479–486
Zielezny Y, Groeneweg J, Vereecken H, Tappe W (2006) Impact of sulfadiazine and chlorotetracycline on soil bacterial community structure and respiratory activity. Soil Biol Biochem 38:2372–2380
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This work was financially supported by National Natural Science Foundation of China (Nos. 51778006 and 41571444).
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Tong, T., Xie, S. Impacts of sulfanilamide and oxytetracycline on methane oxidation and methanotrophic community in freshwater sediment. Ecotoxicology 28, 392–398 (2019). https://doi.org/10.1007/s10646-019-02026-0
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DOI: https://doi.org/10.1007/s10646-019-02026-0