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Silicon-induced antioxidant defense and methylglyoxal detoxification works coordinately in alleviating nickel toxicity in Oryza sativa L.

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Abstract

Nickel (Ni), an essential nutrient of plant but very toxic to plant at supra-optimal concentration that causes inhibition of seed germination emergence and growth of plants as a consequence of physiological disorders. Hence, the present study investigates the possible mechanisms of Ni tolerance in rice seedlings by exogenous application of silicon (Si). Thirteen-day-old hydroponically grown rice (Oryza sativa L. cv. BRRI dhan54) were treated with Ni (NiSO4.7H2O, 0.25 and 0.5 mM) sole or in combination with 0.50 mM Na2SiO3 for a period of 3 days to investigate the effect of Si supply for revoking the Ni stress. Nickel toxicity gave rise to reactive oxygen species (ROS) and cytotoxic methylglyoxal (MG), accordingly, initiated oxidative stress in rice leaves, and accelerated peroxidation of lipids and consequent damage to membranes. Reduced growth, biomass accumulation, chlorophyll (chl) content, and water balance under Ni-stress were also found. However, free proline (Pro) content increased in Ni-exposed plants. In contrast, the Ni-stressed seedlings fed with supplemental Si reclaimed the seedlings from chlorosis, water retrenchment, growth inhibition, and oxidative stress. Silicon up-regulated most of the antioxidant defense components as well as glyoxalase systems, which helped to improve ROS scavenging and MG detoxification. Hence, these results suggest that the exogenous Si application can improve rice seedlings’ tolerance to Ni-toxicity.

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Acknowledgements

The authors acknowledge Mr. Abdul Awal Chowdhury Masud, Department of Agronomy, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh for critical reading and editing of the manuscript.

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  1. These authors contributed equally: Mirza Hasanuzzaman, Md. Mahabub Alam

Authors and Affiliations

  1. Department of Agronomy, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka, 1207, Bangladesh

    Mirza Hasanuzzaman & Md. Mahabub Alam

  2. Department of Agriculture, Faculty of Science, Noakhali Science and Technology University, Noakhali, 3814, Bangladesh

    Md. Mahabub Alam

  3. Department of Agricultural Botany, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka, 1207, Bangladesh

    Kamrun Nahar

  4. Laboratory of Plant Stress Responses, Department of Applied Biological Sciences, Faculty of Agriculture, Kagawa University, Miki-Cho, Kita-Gun, Kagawa, 761-0795, Japan

    Sayed Mohammad Mohsin, M. H. M. Borhannuddin Bhuyan, Khursheda Parvin & Masayuki Fujita

  5. Department of Plant Pathology, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka, 1207, Bangladesh

    Sayed Mohammad Mohsin

  6. Citrus Research Station, Bangladesh Agricultural Research Institute, Jaintapur, Sylhet, Bangladesh

    M. H. M. Borhannuddin Bhuyan

  7. Department of Horticulture, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka, 1207, Bangladesh

    Khursheda Parvin

  8. Department of Plant Physiology, University of Life Sciences in Lublin, Lublin, Poland

    Barbara Hawrylak-Nowak

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  1. Mirza Hasanuzzaman
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Hasanuzzaman, M., Alam, M.M., Nahar, K. et al. Silicon-induced antioxidant defense and methylglyoxal detoxification works coordinately in alleviating nickel toxicity in Oryza sativa L.. Ecotoxicology 28, 261–276 (2019). https://doi.org/10.1007/s10646-019-02019-z

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