Bioaccumulation of sediment-associated substituted phenylamine antioxidants in Tubifex tubifex and Lampsilis siliquoidea
Substituted phenylamine antioxidants (SPAs) are additives in a variety of commercial polymers (e.g., lubricants, plastics, etc.). Based on their physicochemical properties, if SPAs were to enter an aquatic system, they would likely partition into sediment and have the capacity to bioaccumulate in biota. This study investigated the potential of four sediment-associated SPAs, diphenylamine (DPA), N-phenyl-1-naphthalene (PNA), N-(1,3-dimethylbutyl)-N’-phenyl-1,4-phenylenediamine (DPPDA), and 4,4’-methylene-bis[N-sec-butylaniline] (MBA) to accumulate in the tissues of freshwater mussels (Lampsilis siliquoidea) and oligochaete worms (Tubifex tubifex). Mussels and worms were exposed to sediment spiked with individual SPAs for 28 d. The concentration of SPAs was measured in the gill, gonad, and remaining viscera of the mussels and entire body of the worms. The majority of biota-sediment accumulation factors (28-d BSAFs) for the different tissues of mussels were < 1. The highest concentrations of SPAs were consistently observed in the gill tissue of mussels relative to the gonad and viscera. The 28-d BSAFs for DPPDA and MBA for worms were < 1, and for DPA and PNA, they ranged from 0.38–2.13 and 1.54–33.24, respectively. The higher 28-d BSAFs observed for worms compared to mussels were likely because worms are endobenthic and feed on sediment-associated organic matter. PNA and DPPDA have similar octanol-water partition coefficients (Kow) but greater 28-d BSAFs were observed for PNA compared to DPPDA for both species. This observation provides evidence that biota may be able to metabolize and/or excrete SPAs with similar physicochemical properties at considerably different rates. The 28-d BSAFs observed for sediment-associated SPAs are lower than those typically required for a chemical to be classified as bioaccumulative.
KeywordsUnionidae Oligochaete Biota-sediment accumulation factor Freshwater mussel High production volume chemical
The authors would like to thank Jennifer Unsworth for assisting with the experimentation with T. tubifex.
The funding for this study was provided by the Government of Canada’s Chemicals Management Plan.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
This articles does not contain any studies with human participants or animals performed by any of the authors.
- Balakrishnan, V. et al. (2016). Chemicals Management Plan Progress Report—The environmental fate, distribution and effects of substituted phenylamine antioxidants (SPAs). Developing analytical methods, investgiating toxicity and evaluating bioaccumlation. Final Report to Science and Risk Assessment Directorate of Environment and Climate Change Canada. Ottawa, ON: Environment and Climate Change CanadaGoogle Scholar
- ECHA (2008) European Union Risk Assessment Report for diphenylamine. European Chemical Agency, HelsinkiGoogle Scholar
- Folch J et al. (1957) A simple method for the isolation and purification of total lipids from animal tissue. J Biol Chem 226:497–509Google Scholar
- Government of Canada (ed) (1999) Canadian Environmental Protection Act, 1999—Persistence and bioaccumulation regulations SOR/2000-107, Vol. SOR/2000-107. Government of Canada, OttawaGoogle Scholar
- Government of Canada (2014) Overview of the Chemicals Management Plan, Vol. 2016. Government of Canada, Ottawa, ONGoogle Scholar
- Haag W (2012) North American freshwater mussels: natural history. Ecology and conservation. Cambridge University Press, New York, NYGoogle Scholar
- Maier C, Calafut T (1998) Polyproylene: the definitive user’s guide and databook. Plastics Design Library, Norwich, NYGoogle Scholar
- MOECC (2011) Bioaccumulation of Sediment-associated Contaminants in Freshwater Organisms, E3495. Aquatic Toxicology Unit, Toronto, ONGoogle Scholar
- OECD (2004) Screening information data set—initial assessment report—N-(1,3-Dimethylbutyl)-N’-phenyl-1,4-phenylenediamine. Organization for Economic Co-operation and Development, ParisGoogle Scholar
- Prosser, R. et al. (2017b) Effect of sediment-associated substituted phenylamine antioxidants on three life stages of the freshwater mussel Lampsilis siliquoidea. Environ Pollut. https://doi.org/10.1016/j.envpol.2017.05.086
- Prosser, R. et al. (2017c) Toxicity of sediment-associated substituted phenylamine antioxidants on the early-life stages of fathead minnows and a characterization of effect on freshwater organisms. Environ Toxicol Chem. https://doi.org/10.1002/etc.3828
- Sikka H et al. (1981) Environmental fate and effects of N-phenyl-1-naphthylamine and its disposition and metabolism in the rate Report No. AFOSR-TR-81-0703. Syracuse Research Company, Syracuse, NYGoogle Scholar
- USEPA (2012) Estimating persistence, bioaccumulaton, and toxicity using the USEPA PBT profiler. United States Environmental Protection Agency, Washington, DCGoogle Scholar
- WHO (1998) Concise international chemical assessment document 9 N-phenyl-1-napthylamine. In: Koennecker G, Mangelsdorf I, Wibbertmann A (eds). World Health Organization, GenevaGoogle Scholar