Gene expression profile changes in Eisenia fetida chronically exposed to PFOA
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Eisenia fetida is a terrestrial organism, which can be used to diagnose sub-lethal concentrations of PFOA by using molecular biomarkers. In order to identify potential molecular biomarkers, we have exposed E. fetida to 10 mg/kg of PFOA in soil for 8 months. The mRNA isolation, sequencing, transcriptome assembly followed by differential gene expression studies have revealed that genes that are involved in apoptotic process, reproduction, calcium signalling, neuronal development and lipid metabolism are predominantly affected. Highly specific genes that are altered by PFOA can be further validated and used as biomarker to detect sub-lethal concentrations of PFOA in the soil.
KeywordsEisenia fetida Perfluorooctanoic acid (PFOA) Transcriptome assembly Differential gene expression Toxicogenomics
The authors acknowledge the Ramaciotti Centre for Genomics, The University of New South Wales, Sydney for mRNA sequencing and eResearch SA for computing facility. Mrs. Srinithi Mayilswami is a recipient of IPRS and CRCCARE top-up scholarships.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
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