Genetic population structure of polychaeta Neanthes glandicincta (Nereididae) of the Mai Po Inner Deep Bay Ramsar Site, Hong Kong
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Neanthes glandicincta (Nereididae, Polychaeta) is the first numerically dominant benthic infauna in the Mai Po international Ramsar site, Hong Kong and also an economically important species for food source of birds and fishes. In present study, highly conserved nuclear ribosomal DNA (SSU and LSU rDNA) and mitochondrial COI gene were employed to study the population structure of N. glandicincta in the subtropical mudflat. The specimens were collected from five localities in February 2006, February–August 2007 and preserved at −80 °C, methanol or formalin, respectively. DNA extraction efficiency was the highest in fresh materials and lowest in formalin-fixed samples. The 18S (1774 bp), 28S D1 (383 bp) and COI genes were sequenced and analyzed. Both 18S and 28S D1 rDNA were highly conserved and showed no difference among the populations, whereas COI gene exhibited relatively high-level intraspecific polymorphism (2.2 %). The population from onshore and near mangrove station was phylogenetic different from other sites, indicating restricted gene exchange between the region of river mouth and mangrove forest. The mangrove may form a barrier for the dispersal of pelagic/benthic larvae of the population, which indicates that the population genetic difference is related to different habitats.
KeywordsNeanthes glandicincta 18S rDNA 28S rDNA Mitochondrial COI gene Phylogenetic analysis
This study was supported by the Ecological Monitoring Program for the Mai Po and Inner Deep Bay Ramsar Site under the contact No. AFCD/SQ/28/01-07, the Knowledge Innovation Program of the Chinese Academy of Sciences under contract SQ201202 and National Natural Science Foundation of China under contract 41006092 and 41130855. We would like to thank our team members in the Laboratory of Environmental Toxicology, the University of Hong Kong and the field working group for logistic support on sampling transport and assistance.
Conflict of interest
The authors declare that they have no conflict of interest.
- Brusca RC, Brusca GJ (2003) Invertebrates, 2nd edn. Sinauer Associates Inc, SunderlandGoogle Scholar
- Fauchald K (1977) The polychaete worms. Definitions and keys to the orders, families and genera. Nat Hist Mus Los Angel County Sci Ser 28:1–188Google Scholar
- Gambi MC, Castelli A, Giangrande A, Lanera P, Predevelli D, Vandini RZ (1994) Polychaetes of commercial and applied interest in Italy: an overview. Mem Mus Natl Hist Nat 162:593–603Google Scholar
- Gooch L (1975) Mechanisms of evolution and population genetics, Vol 2 (1). In: Kinne O (ed) Marine ecology: a comprehensive, integrated treatise on life in oceans and coastal waters. Wiley, New York, pp 351–409Google Scholar
- Greer CE, Peterson SL, Kiviat NB, Manos MM (1991) PCR amplification from paraffin embedded tissues. Am J Clin Pathol 95:117–124Google Scholar
- Hateley JG, Grant A, Jones NV (1989) Heavy metal tolerance in estuarine populations of Nereis diversicolor. In: Ryland JS, Tyler PA (eds) Reproduction, genetics and distributions of marine organisms. Proceedings of the 23rd European Marine Biology Symposium, School of Biological Sciences, University of Wales, Swansea, Fredensborg, Denmark, pp. 379–385Google Scholar
- Kojima S, Ohta S, Yamamotot T, Yamaguichi T, Miura T, Fujiwara Y, Fuijikura K, Hashimoto J (2003) Molecular taxonomy of vestimentiferans of the western Pacific, and their phylogenetic relationship to species of the eastern Pacific III. Alasia-like vestimentiferans and relationships among families. Mar Biol 142:625–635Google Scholar
- Kruse I, Reusch TBH, Schneider MV (2003) Sibling species or poecilogony in the polychaete Scoloplos armiger? Mar Biol 142:937–947Google Scholar
- Lai MY (2004) Fractionation, mobilization and bioaccumulation of heavy metals and mineralogical characteristics of the Mai Po Inner Deep Bay mudflat. M. Phil. thesis, The University of Hong KongGoogle Scholar
- McHugh D (2001) Molecular phylogenetic analyses indicate a rapid radiation of polychaete annelids. Am Zool 41:1520–1521Google Scholar
- McHugh D (2005) Molecular systematics of polychaetes (Annelida). Hydrobiologia 535:309–318Google Scholar
- Neller RJ, Lam KC (1994) The environment. In: Yeung YM, Chu DKY (eds) Guangdong: survey of a province undergoing rapid change. The Chinese University Press, Hong Kong, pp 401–428Google Scholar
- Polanco C, González AI, Dover GA (2000) Patterns of variation in the intergenic spacers of ribosomal dna in drosophila melanogaster support a model for genetic exchanges during X-Y pairing. Genetics 155:1221–1229Google Scholar
- Reisch DJ (1984) Marine ecotoxicological tests with polychaetous annelids. In: Persoone G, Jaspers E, Claus C (eds). Ecotoxicological testing for the marine environment. vol 1, pp 427–454. State University of Ghent and Institute of Marine Scientific Research, GhentGoogle Scholar
- Shin PKS (2001) Population Dynamics and secondary production of Neanthes glandicincta (Polychaeta: Nereididae) from a subtropical mudflat. Asian Mar Biol 18:117–127Google Scholar
- Wu BL, Sun RP, Yang DJ (1981) The Nereidae (Polychaetous Annelids) of the Chinese Coast, BeijingGoogle Scholar