Effects of enhanced UV-B radiation on the diversity and activity of soil microorganism of alpine meadow ecosystem in Qinghai–Tibet Plateau
- 379 Downloads
The effects of enhanced UV-B radiation on abundance, community composition and the total microbial activity of soil bacteria in alpine meadow ecosystem of Qinghai–Tibet Plateau were investigated. Traditional counting and 16S rRNA gene sequencing were used to investigate the culturable bacteria and their composition in soil, meanwhile the total microbial activity was measured by microcalorimetry. The population of soil culturable bacteria was slightly reduced with the enhanced UV-B radiation in both of the two depths, 2.46 × 106 CFU/g in upper layer (0–10 cm), 1.44 × 106 CFU/g in under layer (10–20 cm), comparing with the control (2.94 × 106 CFU/g in upper layer, 1.65 × 106 CFU/g in under layer), although the difference was not statistically significant (P > 0.05). However, the bacteria diversity decreased obviously due to enhanced UV-B, the number of species for upper layer was decreased from 20 to 13, and from 16 to 13 for the lower layer. The distribution of species was also quite different between the two layers. Another obvious decrease induced by enhanced UV-B radiation was in the total soil microbial activities, which was represented by the microbial growth rate constant (k) in this study. The results indicated that the culturable bacteria community composition and the total activity of soil microbes have been considerably changed by the enhanced UV-B radiation.
KeywordsQinghai–Tibet Plateau UV-B radiation Soil microbes Ozone depletion Microcalorimetry
This research was supported by National Basic Research Program (2012CB026105), National Natural Science Foundation (31170482, 31370450), PhD Programs Foundation of Ministry of Education (2010021111002, 20110211110021), The Fundamental Research Funds for the Central Universities (LZUJBKY-2013-92) in China, and State Key Laboratory of Frozen Soil Engineering, Chinese Academy of Sciences (SKLFSE200901). We are grateful to Dr Yantian Ma for his help.
Conflict of interest
The authors declare that they have no conflict of interest.
- Bao X, Li Q, Hua J, Zhao T, Liang W (2013) Interactive effects of elevated ozone and UV-B radiation on soil nematode diversity. Ecotoxicology 23(1):11–20Google Scholar
- Lane DJ (1991) 16S/23S rRNA sequencing. In: Stackebrandt E, Goodfellow M (eds) Nucleic acid techniques in bacterial systematics. Wiley, New York, pp 115–148Google Scholar
- Rex M, Salawitch R, von der Gathen P, Harris N, Chipperfield M, Naujokat B (2004) Arctic ozone loss and climate change. Geophys Res Lett 31(4):L04116. doi: 10.1029/2003GL018844
- Rinnan R, Keinänen M, Kasurinen A, Asikainen J, Kekki T, Holopainen T, Ro-Poulsen H, Mikkelsen TN, Michelsen A (2005) Ambient ultraviolet radiation in the Arctic reduces root biomass and alters microbial community composition but has no effects on microbial biomass. Glob Change Biol 11(4):564–574CrossRefGoogle Scholar
- Russell JM, Luo MZ, Cicerone RJ, Deaver LE (1996) Satellite confirmation of the dominance of chlorofluorocarbons in the global stratospheric chlorine budget. Nature 379:526–529Google Scholar
- Sandmann G, Kuhn S, Böger P (1998) Evaluation of Structurally Different Carotenoids inEscherichia coli Transformants as Protectants against UV-B Radiation. Appl Environ Microbiol 64(5):1972–1974Google Scholar
- Suurkuusk J, Wadsö I (1982) A multichannel microcalorimetry system. Chem Scr 20:155–163Google Scholar
- Zhou J, Bruns M, Tiedje J (1996) DNA recovery from soils of diverse composition. Appl Environ Microbiol 62:316–322Google Scholar
- Zhou X, Luo C, Li W, Shi J (1995) Changes of total ozone in whole China and its low contents center in Qing-Zang Plateau regions. Chin Sci Bull 40:1396–1398Google Scholar