Evaluating pyrene toxicity on Arctic key copepod species Calanus hyperboreus
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Calanus hyperboreus is a key species in the Arctic regions because of its abundance and role in the Arctic food web. Exploitation of the off shore oil reserves along Western Greenland is expected in the near future, and it is important to evaluate the acute and chronic effects of oil emissions to the ecosystem. In this study C. hyperboreus females were exposed to concentrations of 0, 0.1, 1, 10 and 100 nM pyrene and saturated concentrations measured to ~300 nM. Daily quantification of egg and faecal pellet production showed significant decreases in the pellet production, while the egg production was unaffected. The hatching success was also unaffected, although the total reproductive output was reduced with increased pyrene concentrations. Accumulation of pyrene in the copepods was higher in feeding than starving females and only trace amounts of the phase I metabolite 1-hydroxypyrene, were found. Lowered reproductive output, reduced grazing, and reduced ability to metabolize pyrene suggest that oil contamination may constitute a risk to C. hyperboreus recruitment, energy transfer in the food web and transfer of pyrene to higher trophic levels.
KeywordsCalanus hyperboreus PAH Pyrene Faecal pellet production Egg production
This study was funded by the Carlsberg Foundation, Greenland Climate Research Center (GCRC Grant 6505), Bureau of Minerals and Petroleum, Greenland, Selskabet for Arktisk Forskning og Teknologi, Knud Højgårds Fond and the Oticon Foundation. The fieldwork took place at Arctic Station (University of Copenhagen, Qerqertarsuaq)—a big thanks to the station manager Ole Stecher and staff and to the crew of RV Porsild for providing a great working environment. We would especially like to thank Abel Brandt and Johannes Mølgaard, who with their hard work, good spirits and limitless knowledge on local conditions made our work on the sea ice possible.
Conflict of interest
The authors declare that they have no conflict of interest.
- AMAP (2007) Arctic oil and gas 2007. In: Arctic monitoring and assessment programme, OsloGoogle Scholar
- Diercks AR, Highsmith RC, Asper VL, Joung D, Zhou Z, Guo L, Shiller AM, Joye SB, Teske AP, Norman G, Wade TL, Lohrenz SE (2010) Characterization of subsurface polycyclic aromatic hydrocarbons at the deepwater horizon site. Geophys Res Lett 37(20):L20602Google Scholar
- Falk-Petersen S, Timofeev S, Pavlov V, Sargent JR (2006) Climate variability and possible effects on arctic food chains: the role of Calanus. In: Ørbaek JB et al (eds) Arctic alpine ecosystems and people in a changing environment. Springer, BerlinGoogle Scholar
- Hydrocarbonstrategy (2009) Exploration and exploitation of hydrocarbons in Greenland. Bureau of minerals and petroleum, the Greenland home rule government, NuukGoogle Scholar
- Jung-Madsen S, Nielsen TG, Grønkjær P, Hansen BW, Møller EF (2013) Early development of Calanus hyperboreus nauplii—Response to a changing ocean. Limnol Oceanog 58:2109–2121Google Scholar
- Oil in the Sea III (2003) Oil in the sea III: inputs, fates and effects. The National Academies Press, WashingtonGoogle Scholar
- Reigstad M, Riser C, Svenson C (2005) Fate of copepod faecal pellets and the role of Oithona spp. Mar Ecol Prog Ser 304:265-270Google Scholar
- Rewitz KF, Styrishave B, Lobner-Olesen A, Andersen O (2006) Marine invertebrate cytochrome P450: emerging insights from vertebrate and insect analogies. Comp Biochem Physiol Part C 143:363–381Google Scholar
- Socio-economic aspects (2004) Report on socio-economic aspects of hydrocarbon and exploitation and exploration in Greenland. Bureau of Minerals and Petroleum, the Greenland Home Rule Government, NuukGoogle Scholar
- Statistics Greenland (2012) Grønlands Udenrigshandel 2011, vol 1. Udenrigshandel, Nuuk, p 1–19Google Scholar
- Swalethorp R, Kjellerup S, Dünweber M, Nielsen TG, Møller EF, Rysgaard S, Hansen BW (2011) Grazing, egg production, and biochemical evidence of differences in the life strategies of Calanus finmarchicus, C. glacialis and C. hyperboreus in Disko Bay, western Greenland. Mar Ecol Prog Ser 429:125–144CrossRefGoogle Scholar
- Varanasi U (1989) Metabolism of polycyclic hydrocarbons in the aquatic environments. CRC Press, Boca Ranton, FLGoogle Scholar
- Welch HE, Bergmann MA, Siferd TD, Martin KA, Curtis MF, Crawford RE, Conover RJ, Hob H (1992) Energy flow through the marine ecosystem of the Lancaster Sound region, arctic Canada. Arctic 45:343–357Google Scholar