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Ecotoxicology

, Volume 20, Issue 8, pp 1890–1899 | Cite as

Genotoxicity in Atlantic killifish (Fundulus heteroclitus) from a PAH-contaminated Superfund site on the Elizabeth River, Virginia

  • Dawoon Jung
  • Cole W. Matson
  • Leonard B. Collins
  • Geoff Laban
  • Heather M. Stapleton
  • John W. Bickham
  • James A. Swenberg
  • Richard T. Di Giulio
Article

Abstract

The Atlantic Wood Industries Superfund site (AWI) on the Elizabeth River in Portsmouth, VA is heavily contaminated with polycyclic aromatic hydrocarbons (PAHs) from a wood treatment facility. Atlantic killifish, or mummichog (Fundulus heteroclitus), at this Superfund site are exposed to very high concentrations of several carcinogens. In this study, we measured PAH concentrations in both fish tissues and sediments. Concurrently, we assessed different aspects of genotoxicity in the killifish exposed in situ. Both sediment and tissue PAH levels were significantly higher in AWI samples, relative to a reference site, but the chemistry profile was different between sediments and tissues. Killifish at AWI exhibited higher levels of DNA damage compared to reference fish, as measured via the flow cytometric method (FCM), and the damage was consistent with sediment PAH concentrations. Covalent binding of benzo[a]pyrene (BaP) metabolites to DNA, as measured via LC–MS/MS adduct detection methods, were also elevated and could be partially responsible for the DNA damage. Using similar LC–MS/MS methods, we found no evidence that oxidative DNA adducts had a role in observed genotoxicity.

Keywords

Fundulus heteroclitus DNA adduct Chromosomal damage Elizabeth River Atlantic Wood Industries Superfund site Polycyclic aromatic hydrocarbon Biomarker 

Notes

Acknowledgments

We thank Drs. Lauren Wills and Bryan Clark, and Lindsey Van Tiem for assistance in sample collection and processing, and Shannon Kelly, Sara Eagle, Dr. Ben Colman for technical support for sediment and tissue analysis. Standards for BPDE adduct analysis were provided by Dr. Natalia Tretyakova of University of Minnesota. This research was funded by Duke Superfund Research Program (P42 ES10356), Duke Integrated Toxicology and Environmental Health Program (NIEHS, T32ES07031), and UNC Superfund Research Program (P42 ES05948).

Supplementary material

10646_2011_727_MOESM1_ESM.pdf (44 kb)
Supplementary material 1 (PDF 44 kb)

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Dawoon Jung
    • 1
    • 2
  • Cole W. Matson
    • 1
    • 3
  • Leonard B. Collins
    • 4
  • Geoff Laban
    • 5
  • Heather M. Stapleton
    • 1
  • John W. Bickham
    • 5
  • James A. Swenberg
    • 4
  • Richard T. Di Giulio
    • 1
  1. 1.Nicholas School of the EnvironmentDuke UniversityDurhamUSA
  2. 2.Department of Microbiology and ImmunologyDartmouth Medical SchoolHanoverUSA
  3. 3.Center for the Environmental Implications of NanoTechnology (CEINT)Duke UniversityDurhamUSA
  4. 4.Center for Environmental Health and SusceptibilityGillings School of Global Public Health, University of North CarolinaChapel HillUSA
  5. 5.Center for the EnvironmentPurdue UniversityW. LafayetteUSA

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