, 20:1018 | Cite as

Toxicity of cyanobacterial bloom extracts from Taihu Lake on mouse, Mus musculus

  • Dingsheng Li
  • Zhengtao Liu
  • Yibin Cui
  • Wenlong Li
  • Hao Fang
  • Mei Li
  • Zhiming Kong


The acute and sub-chronic toxicities of cyanobacterial extract from Taihu Lake (PR China) on mouse (Mus musculus) were investigated in this study via intraperitoneal (i.p.) injection. Increases in liver/body weight ratios and pathological changes in mouse liver showed adverse effects at the organ level. Images from transmission electron microscopy (TEM) indicated that abnormal membrane structure occurred and that the organelles were damaged severely in the cells of liver and testis. The high dose group received i.p. injection of 12 mg lyophilized algae cells/kg body weight. Malondialdehyde (MDA) levels increased significantly in the livers of this group, along with a significant decrease in catalase (CAT) activity. These results revealed the existence of obvious oxidative stress. Comet assay results also suggested a dose-dependent relationship between DNA damage in hepatocytes/testicular cells and the amount of bloom extract administered to the mice. There was a significant increase in DNA damage compared to the control group and the genotoxicity of the cyanobacterial bloom to testicular cells was higher than in hepatocytes.


Cyanobacterial bloom extract Genotoxicity Malondialdehyde Catalase Comet assay 



This work was supported by project of the 973 National Basic Research Program of China (No. 2008CB418103 and 2008CB418102) and the National Major Project of Science & Technology Ministry of China (No. 2008ZX07526-003 and 2008ZX07101-004). We also want to express gratefulness to Prof. Xiaorong Wang and Dr. Jinlin Jiang of Nanjing University for their assistance in the HPLC analysis.


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Dingsheng Li
    • 1
  • Zhengtao Liu
    • 2
  • Yibin Cui
    • 1
  • Wenlong Li
    • 1
  • Hao Fang
    • 1
  • Mei Li
    • 1
  • Zhiming Kong
    • 1
  1. 1.State Key Laboratory of Pollution Control and Resource Reuse, School of the EnvironmentNanjing UniversityNanjingPeople’s Republic of China
  2. 2.Key Laboratory for Ecology Effect and Risk Assessment of Chemicals, MEPChinese Research Academy of Environment ScienceBeijingPeople’s Republic of China

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