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Ecotoxicology

, Volume 18, Issue 8, pp 974–982 | Cite as

Juvenile sea bass (Dicentrarchus labrax L.) enzymatic and non-enzymatic antioxidant responses following 17β-estradiol exposure

  • Iqbal Ahmad
  • Vera Lúcia Maria
  • Mário Pacheco
  • Maria Ana Santos
Article

Abstract

In the context of 17β-estradiol (E2) as an environmental contaminant, this study was designed to test the hypothesis whether it can modulate antioxidant defenses in Dicentrarchus labrax, taking gills as the target organ. Enzymatic (GPX−glutathione peroxidase; CAT-catalase; GR-glutathione reductase; GST-glutathione S-transferase) and non-enzymatic antioxidants (NP-SH-non protein thiols; GSHt-total glutathione) were measured following 10-day exposure to E2 in two different ways: water diluted (WD, 200 or 2,000 ng/L) and intraperitoneally injected (IP, 0.5 or 5 mg/kg). WD exposure caused a single alteration—CAT increase, whereas IP exposure decreased all the enzymatic antioxidants. Similarly, NP-SH and GSHt were reduced by IP exposure. Thus, different E2 exposure routes determined clear differences on the assessed responses. Despite gills close contact with water, their defenses were not strongly affected in WD experiment. Differently, IP injected fish showed an overall decrease in both enzymatic and non-enzymatic antioxidants, more pronounced at the highest concentration, pointing out the E2 oxidative stress inducing potential in fish.

Keywords

Juvenile Dicentrarchus labrax L. Oxidative stress Gills antioxidants 17β-Estradiol 

Notes

Acknowledgments

The financial supports from FCT (Government of Portugal) provided through POCI/MAR/61195/2004 SFRH/ BPD/ 34326/ 2006, SFRH/ BPD/ 26970/ 2006 and by the Aveiro University Research Institute/CESAM are gratefully acknowledged.

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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Iqbal Ahmad
    • 1
  • Vera Lúcia Maria
    • 2
  • Mário Pacheco
    • 1
  • Maria Ana Santos
    • 1
  1. 1.CESAM & Department of BiologyUniversity of AveiroAveiroPortugal
  2. 2.CIMA, Faculty of Marine and Environmental SciencesUniversity of AlgarveFaroPortugal

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