Abstract
There has been very little effort to understand genetic divergence between wild and hatchery populations of masu salmon (Oncorhynchus masou). In this study, we used mitochondrial (mt) NADH dehydrogenase subunit 5 gene (ND5) and six polymorphic nuclear microsatellite DNA loci to compare the genetic variability in three hatchery broodstocks of masu salmon with the variability in eight putative wild masu populations sampled in five rivers including one known source river for the hatchery broodstocks. Both ND5 and microsatellites showed no significant genetic divergence (based on FST estimates) between four annual collections from the source river population, suggesting no change in genetic diversity over this time period. The FST estimates, an analysis of molecular variance (AMOVA), and a neighbor-joining tree using both DNA markers suggested significant differentiation between the three hatchery and all eight putative wild populations. We conclude that genetic diversity of hatchery populations are low relative to putative wild populations of masu salmon, and we discuss the implications for conservation and fisheries management in Hokkaido.
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Acknowledgments
This study was supported in part by Grants-in-Aid from the Fisheries Agency of Japan, the Northern Advancement Center for Science and Technology, and the 21st COE program (K-2) ‘Marine Bio-Manipulation Frontier for Food Production’ at Hokkaido University, sponsored by the Ministry of Education, Culture, Sports, Science and Technology, Japan.
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Appendix. Allelic frequencies of the six microsatellite DNA loci in wild and hatchery collections. Population codes are listed in Table 1
Appendix. Allelic frequencies of the six microsatellite DNA loci in wild and hatchery collections. Population codes are listed in Table 1
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Yu, JN., Azuma, N. & Abe, S. Genetic differentiation between collections of hatchery and wild masu salmon (Oncorhynchus masou) inferred from mitochondrial and microsatellite DNA analyses. Environ Biol Fish 94, 259–271 (2012). https://doi.org/10.1007/s10641-011-9869-0
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DOI: https://doi.org/10.1007/s10641-011-9869-0