Natural triploidy in Rhamdia quelen identified by cytogenetic monitoring in Iguaçu basin, southern Brazil

  • Maelin da Silva
  • Daniele A. Matoso
  • Luis Augusto M. Ludwig
  • Eder Gomes
  • Mara C. Almeida
  • Marcelo R. Vicari
  • Roberto Ferreira Artoni


The freshwater silver catfish from the genus Rhamdia belongs to a group of neotropical fish with wide distribution throughout Central and South America. The results obtained in the F1 generation of native specimens of this species bred in captivity using molecular cytogenetic tools revealed a high number of triploid specimens (46.8%) in the samples analyzed. The use of double fluorescent in situ hybridization (double-FISH) with 18S and 5S rDNA probes allowed highlighting the location of ribosomal sites with independent activity. This chromosome marker allowed the identification of triploid nuclei, in which three markers were visualized, in contrast to diploid fish, which only exhibited two fluorescent signals. As triploid specimens exhibit either total or partial gonad sterility, the present study demonstrates the importance of cytogenetic monitoring in restocking programs aimed at genetic conservation.


Conservation programs Genetic conservation Rhamdia Triploidy 



The authors are grateful to the Instituto Ambiental do Paraná and the Instituto Brasileiro do Meio Ambiente e Recursos Naturais Renováveis for authorizing the capture of the specimens (IBAMA/MMA/SISBIO license number: 15117). This study was supported by the Brazilian fostering agencies Conselho Nacional de Desenvolvimento Científico e Tecnológico (process: 473448/2007-6), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior and Fundação Araucária de Apoio ao Desenvolvimento Científico e Tecnológico do Estado do Paraná (process: 12938). We also thank Mr. Miguel Airton Carvalho for collaboration in the field and laboratory activities.


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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Maelin da Silva
    • 1
  • Daniele A. Matoso
    • 2
  • Luis Augusto M. Ludwig
    • 3
  • Eder Gomes
    • 3
  • Mara C. Almeida
    • 1
  • Marcelo R. Vicari
    • 1
  • Roberto Ferreira Artoni
    • 1
  1. 1.Departamento de Biologia Estrutural Molecular e Genética, Laboratório de Citogenética e EvoluçãoUniversidade Estadual de Ponta GrossaPonta GrossaBrazil
  2. 2.Instituto Nacional de Pesquisas da AmazôniaManausBrazil
  3. 3.Estação de Hidrobiologia da Usina de Salto Segredo, COPELMangueirinhaBrazil

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