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The in vitro assessment of dipyridophenazine complexes in H-ras oncogene transformed rat embryo fibroblast 5RP7 cell line

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Summary

Purpose The aim of this study is to detect apoptotic and cytotoxic/antiproliferative effects of a ligand substance and its metal derivatives. The substances were investigated by using an h-ras oncogene transformed rat embryo fibroblast cell line (5RP7). Methods The cytotoxic influences of dipyrido[3,2-a:2′,3’c]phenazine ligand, dipyrido[3,2-a:2′,3’c] phenazine-platinum(II) complex ([Pt(dppz)Cl2]) and dipyrido[3,2-a:2′,3’c] phenazine-gold(III) complex ([Au(dppz)Cl2]Cl) were determined with MTT (3[4,5-dimetiltiyazol2-yl]-2,5-difeniltetrazolyum bromid) assay on 5RP7 cells. Results Dipyrido[3,2-a:2′,3’c] phenazine, dipyrido[3,2-a:2′,3’c] phenazine-platinum(II) complex ([Pt(dppz)Cl2]) and dipyrido[3,2-a:2′,3’c] phenazine-gold(III) complexes ([Au(dppz)Cl2]Cl) caused significant increase in cytotoxicity in a dose and time dependent manner. The effects of dipyridophenazine ligand (dppz) and its metal derivatives on apoptosis were monitorized using cytotoxic dose (10 μM) DAPI fluorescent staining. It was shown that dppz and its compounds induced apoptosis. Conclusions These findings show that dpzz and its complexes can be studied as novel alternative chemotherapeutics in cancer treatment.

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Acknowledgements

This work was financed by a grant from the Scientific and Technological Research Council of Turkey (TUBITAK-SBAG-108S206). The authors are grateful to Anadolu University and Bezmialem University. We would like to thank Barlas Benkli due to contributions related to corrections of english language.

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Authors and Affiliations

  1. Department of Biology, Faculty of Science, Anadolu University, 26470, Eskisehir, Turkey

    Ayse Kaplan & Ayse Tansu Koparal

  2. Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Bezmialem University, 34093, İstanbul, Turkey

    Kadriye Benkli

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  1. Ayse Kaplan
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Correspondence to Ayse Kaplan.

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Kaplan, A., Benkli, K. & Koparal, A.T. The in vitro assessment of dipyridophenazine complexes in H-ras oncogene transformed rat embryo fibroblast 5RP7 cell line. Invest New Drugs 36, 755–762 (2018). https://doi.org/10.1007/s10637-017-0559-4

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