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Investigational New Drugs

, 27:517 | Cite as

Boldine: a potential new antiproliferative drug against glioma cell lines

  • Daniéli Gerhardt
  • Ana Paula Horn
  • Mariana Maier Gaelzer
  • Rudimar Luiz Frozza
  • Andrés Delgado-Cañedo
  • Alessandra Luiza Pelegrini
  • Amélia T. Henriques
  • Guido Lenz
  • Christianne SalbegoEmail author
PRECLINICAL STUDIES

Summary

Malignant gliomas are the most common and devastating primary tumors of the central nervous system. Currently no efficient treatment is available. This study evaluated the effect and underlying mechanisms of boldine, an aporphine alkaloid of Peumus boldus, on glioma proliferation and cell death. Boldine decreased the cell number of U138-MG, U87-MG and C6 glioma lines at concentrations of 80, 250 and 500 μM. We observed that cell death caused by boldine was cell-type specific and dose-dependent. Exposure to boldine for 24 h did not activate key mediators of apoptosis. However, it induced alterations in the cell cycle suggesting a G2/M arrest in U138-MG cells. Boldine had no toxic effect on non-tumor cells when used at the same concentrations as those used on tumor cells. Based on these results, we speculate that boldine may be a promising compound for evaluation as an anti-cancer agent.

Keywords

Glioma Proliferation Boldine Anti-tumor agent 

Notes

Acknowledgments

This work was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). The authors thank Alessandra Heizelmann for the excellent technical assistance.

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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Daniéli Gerhardt
    • 1
  • Ana Paula Horn
    • 1
  • Mariana Maier Gaelzer
    • 1
  • Rudimar Luiz Frozza
    • 1
  • Andrés Delgado-Cañedo
    • 2
  • Alessandra Luiza Pelegrini
    • 3
  • Amélia T. Henriques
    • 4
  • Guido Lenz
    • 3
  • Christianne Salbego
    • 1
    Email author
  1. 1.Programa de Pós graduação em Ciências Biológicas: Bioquímica, Departamento de Bioquímica, Instituto de Ciências Básicas da SaúdeUFRGSPorto AlegreBrazil
  2. 2.Laboratório de Cardiologia Molecular e CelularIC/FUC-RSPorto AlegreBrazil
  3. 3.Departamento de Biofísica, Instituto de BiociênciasUFRGSPorto AlegreBrazil
  4. 4.Departamento de Produção de Matéria Prima, Faculdade de FarmáciaUFRGSPorto AlegreBrazil

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