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Investigational New Drugs

, Volume 27, Issue 4, pp 297–303 | Cite as

Is transketolase like 1 a target for the treatment of differentiated thyroid carcinoma? A study on thyroid cancer cell lines

  • Eleonore Fröhlich
  • Inge Fink
  • Richard WahlEmail author
PRECLINICAL STUDIES

Summary

Radioactive iodine-refractory [18F] fluorodeoxy-glucose-positron emission tomography-positive thyroid carcinomas represent especially aggressive tumors. Targeting glucose metabolism by the transketolase isoenzyme transketolase like 1 (TKTL-1) which is over-expressed in various neoplasms, may be effective. The correlation of TKTL-1 expression and the response to oxythiamine as the currently best-characterized inhibitor of transketolases was studied in differentiated thyroid cancer cell lines. We determined TKTL-1 expression, proliferation, glucose uptake and GLUT-1 expression in non-treated thyroid cells and recorded the effect of oxythiamine on iodide uptake and on thymidine uptake. TKTL 1 was highest expressed in cell lines derived from more invasive tumors but the expression level was not strongly correlated to proliferation rate, to GLUT-1 expression or to the response to oxythiamine. Oxythiamine showed only a weak effect in the TKTL-1 expressing cell lines. Over-expression of TKTL-1 is not an indicator for responsiveness to oxythiamine. More specific inhibitors should be tested.

Keywords

Thyroid carcinoma Transketolase like 1 Glucose metabolism Glucose transporter Oxythiamine 

Notes

Acknowledgements

The excellent technical help of Mrs Elke Maier is gratefully acknowledged.

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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  1. 1.Department of Endocrinology, Metabolism, Nephrology and Clinical Chemistry, Internal MedicineUniversity of TuebingenTuebingenGermany
  2. 2.Department IV, Internal MedicineUniversity of TuebingenTuebingenGermany

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