Proteomic analysis of prodigiosin-induced apoptosis in a breast cancer mitoxantrone-resistant (MCF-7 MR) cell line
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Prodigiosin (PG) is a bacterial, red-pigmented antibiotic with immunosuppressive and apoptotic activities. To better understand its mechanisms of action, we tried to identify proteins associated with apoptosis induced by PG. For this purpose, the variation of protein expression on exposure to apoptotic concentrations of PG was examined, by high-resolution two-dimensional gel electrophoresis (2D-E), in the MCF-7 cancer cell line resistant to mitoxantrone (MCF-7-MR). Six PG apoptosis-associated protein spots were further characterized by complementary peptide mass fingerprinting and tandem mass spectrometry data obtained on a matrix-assisted laser desorption ionization-time-of-flight/time-of-flight (MALDI-TOF/TOF) mass spectrometer. The proteins identified were involved in various cellular functions, including cell defence, DNA repair and cellular organization. Our data provide novel information on cell response to PG, a new apoptotic drug with interesting anticancer activity.
KeywordsApoptosis Prodigiosin Proteomics Breast cancer
matrix-assisted laser desorption ionization-time-of-flight/time-of-flight
MCF-7 breast cancer cell line resistant to mitoxantrone
mitogen-activated protein kinase
multidrug resistance protein
two-dimensional gel electrophoresis
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We want to thank Miguel Abal for critical and comprehensive reading of the manuscript. We also want to thank Dr. Eliandre de Oliveira and David Bellido from “Plataforma de Proteòmica” University of Barcelona for technical support. M. Monge was a recipient of a fellowship from the University of Barcelona.
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