Suppression of primary breast, colon, gastric and bladder cancers cell growth in vitro by CKBM, a natural product
- 60 Downloads
CKBM is a product composed of natural ingredients and had been shown to possess certain anti-cancer effects in vitro and in vivo. The aim of the present study is to analyze the chemosensitivity in the treatment of primary colon, breast, gastric and bladder cancer cells by CKBM. A total of 77 patients with cancers of breast, colon, stomach or bladder were included in the present study. Primary cancer cells were isolated from the surgical removed tumors and treated with various dosages of CKBM for 5 days. ATP is then extracted and measured by luminescence assay. CKBM treatment inhibited primary colon, breast, gastric and bladder cancer growth dose-dependently. The IC values were smaller from tumor cells at early stages, when compared with the ones at later stages. The present study strongly indicated that CKBM exerted cytotoxic effect on primary cancer cells.
Key Wordschemosensitivity colon cancer gastric cancer breast cancer bladder cancer
Unable to display preview. Download preview PDF.
- 1.Whitehouse PA, Knight LA, Di Nicolantonio F, Mercer SJ, Sharma S, Cree IA: Portsmouth colorectal cancer multidisciplinary team: Heterogeneity of chemosensitivity of colorectal adenocarcinoma determined by a modified ex vivo ATP-tumor chemosensitivity assay (ATP-TCA). Anticancer Drugs 14: 369–375, 2003CrossRefPubMedGoogle Scholar
- 5.Chan JY, Cheung JY, Luk SC, Wu YJ, Pang SF, Fung KP: Anti-cancer and pro-apoptotic effects of an herbal medicine and Saccharomyces cerevisiae product (CKBM) on human hepatocellular carcinoma hep G2 cells in vitro and in vivo. Immunopharmacol Immunotoxicol (accepted)Google Scholar
- 7.Andreotti PE, Cree IA, Kurbacher CM, Hartmann DM, Linder D, Harel G, Gleiberman I, Caruso PA, Ricks SH, Untch M, Sartori C, Bruckner HW: Chemosensitivity testing of human tumors using a microplate adenosine triphosphate luminescence assay: Clinical correlation for cisplatin resistance of ovarian carcinoma. Cancer Res 55: 5276–5282, 1995PubMedGoogle Scholar
- 8.Kuzmits R, Aiginger P, Muller MM, Steurer G, Linkesch W: Assessment of the sensitivity of leukaemic cells to cytotoxic drugs by bioluminescence measurement of ATP in cultured cells. Clin Sci (Lond) 71: 81–88, 1986Google Scholar
- 23.Chen J, Tan KP, Ward WE, Thompson LU: Exposure to flaxseed or its purified lignan during suckling inhibits chemically induced rat mammary tumorigenesis. Exp Biol Med (Maywood) 228: 951–958, 2003Google Scholar