Advertisement

Documenta Ophthalmologica

, Volume 115, Issue 3, pp 203–209 | Cite as

Functional study in NSE-Hu-Bcl-2 transgenic mice: a model for retinal diseases starting in Müller cells

  • Cécile Péant
  • André Dosso
  • Lorenza Eder-Colli
  • Florence Chiodini
Original Research Article
  • 83 Downloads

Abstract

In NSE-Hu-Bcl-2 transgenic mice, line 71, retina undergoes early postnatal degeneration linked to the prior death of Müller cells. The purpose of this study was to complete the characterization of this retinal dysfunction by using electroretinographic (ERG) recordings in both scotopic and photopic conditions. Here, we showed that both rod and cone systems were profoundly affected in NSE-Hu-Bcl-2 transgenic mice as soon as 15 postnatal days in accordance with histological study performed previously.

Keywords

Bcl-2 over-expression Electroretinogram Müller cells Photoreceptors Retinal degeneration 

Abbreviations

cds/m2

Candelas*second/m2

ERG

Electroretinography

IT

Implicit time

MCs

Müller glial cells

NSE-Hu-Bcl-2 mouse

Mouse over-expressing the human Bcl-2 under the control of neuron-specific enolase promotor

OPs

Oscillatory potentials

PN

Postnatal day

WT

Wild-type

tg

Transgenic

Notes

Aknowledgements

This work was supported by the Provisu Foundation, Schmidheiny Foundation, De Reuter Foundation and Novartis Foundation grants to L.E-C.

References

  1. 1.
    Kawasaki A, Otori Y, Barnstable CJ (2000) Müller cell protection of rat retinal ganglion cells from glutamate and nitric oxide neurotoxicity. Invest Ophthalmol Vis Sci 41:3444–3450PubMedGoogle Scholar
  2. 2.
    Reichenbach A, Fuchs U, Kasper M, el Hifnawi E, Eckstein AK (1995) Hepatic retinopathy: morphological features of retinal glial (Müller) cells accompanying hepatic failure. Acta Neuropathol (Berl) 90:273–281CrossRefGoogle Scholar
  3. 3.
    Maw MA, Kennedy B, Knight A, Bridges R, Roth KE, Mani EJ, Mukkadan JK, Nancarrow D, Crabb JW, Denton MJ (1997) Mutation of the gene encoding cellular retinaldehyde-binding protein in autosomal recessive retinitis pigmentosa. Nat Genet 17:198–200PubMedCrossRefGoogle Scholar
  4. 4.
    Morimura H, Berson EL, Dryja TP (1999) Recessive mutations in the RLBP1 gene encoding cellular retinaldehyde-binding protein in a form of retinitis punctata albescens. Invest Ophthalmol Vis Sci 40:1000–1004PubMedGoogle Scholar
  5. 5.
    Saari JC, Crabb JW (2005) Focus on molecules: cellular retinaldehyde-binding protein (CRALBP). Exp Eye Res 81:245–246PubMedGoogle Scholar
  6. 6.
    Bringmann A, Reichenbach A (2001) Role of Müller cells in retinal degenerations. Front Biosci 6:E72–E92PubMedCrossRefGoogle Scholar
  7. 7.
    Bringmann A, Pannicke T, Grosche J, Francke M, Wiedemann P, Skatchkov SN, Osborne NN, Reichenbach A (2006) Müller cells in the healthy and diseased retina. Prog Retin Eye Res 25:397–424PubMedCrossRefGoogle Scholar
  8. 8.
    Li Q, Puro DG (2002) Diabetes-induced dysfunction of the glutamate transporter in retinal Müller cells. Invest Ophthalmol Vis Sci 43:3109–3116PubMedGoogle Scholar
  9. 9.
    Lieth E, Barber AJ, Xu B, Dice C, Ratz MJ, Tanase D, Strother JM (1998) Glial reactivity and impaired glutamate metabolism in short-term experimental diabetic retinopathy. Penn State Retina Research Group. Diabetes 47:815–820PubMedCrossRefGoogle Scholar
  10. 10.
    Rungger-Brändle E, Dosso AA, Leuenberger PM (2000) Glial reactivity, an early feature of diabetic retinopathy. Invest Ophthalmol Vis Sci 41:1971–1980PubMedGoogle Scholar
  11. 11.
    Martinou JC, Dubois-Dauphin M, Staple JK, Rodriguez I, Frankowski H, Missotten M, Albertini P, Talabot D, Catsicas S, Pietra C (1994) Overexpression of BCL-2 in transgenic mice protects neurons from naturally occurring cell death and experimental ischemia. Neuron 13:1017–1030PubMedCrossRefGoogle Scholar
  12. 12.
    Dubois-Dauphin M, Poitry-Yamate C, de Bilbao F, Julliard AK, Jourdan F, Donati G (2000) Early postnatal Müller cell death leads to retinal but not optic nerve degeneration in NSE-Hu-Bcl-2 transgenic mice. Neuroscience 95:9–21PubMedCrossRefGoogle Scholar
  13. 13.
    Bonaventure N, Karli P (1968) Maturation of ERG and evoked visual potentials in mice. C R Seances Soc Biol Fil 162:553–555PubMedGoogle Scholar
  14. 14.
    el Azazi M, Wachtmeister L (1990) The postnatal development of the oscillatory potentials of the electroretinogram. I. Basic characteristics. Acta Ophthalmol (Copenh) 68:401–409CrossRefGoogle Scholar
  15. 15.
    Lyubarsky AL, Pugh EN Jr (1996) Recovery phase of the murine rod photoresponse reconstructed from electroretinographic recordings. J Neurosci 16:563–571PubMedGoogle Scholar
  16. 16.
    Wachtmeister L (1998) Oscillatory potentials in the retina: what do they reveal. Prog Retin Eye Res 17:485–521PubMedCrossRefGoogle Scholar
  17. 17.
    Jaissle GB, May CA, Reinhard J, Kohler K, Fauser S, Lutjen-Drecoll E, Zrenner E, Seeliger MW (2001) Evaluation of the rhodopsin knockout mouse as a model of pure cone function. Invest Ophthalmol Vis Sci 42:506–513PubMedGoogle Scholar
  18. 18.
    Peachey NS, Goto Y, al Ubaidi MR, Naash MI (1993) Properties of the mouse cone-mediated electroretinogram during light adaptation. Neurosci Lett 162:9–11PubMedCrossRefGoogle Scholar
  19. 19.
    Fulton AB, Hansen RM, Findl O (1995) The development of the rod photoresponse from dark-adapted rats. Invest Ophthalmol Vis Sci 36:1038–1045PubMedGoogle Scholar
  20. 20.
    Kainz PM, Adolph AR, Wong KY, Dowling JE (2003) Lazy eyes zebrafish mutation affects Müller glial cells, compromising photoreceptor function and causing partial blindness. J Comp Neurol 463:265–280PubMedCrossRefGoogle Scholar
  21. 21.
    Peachey NS, Quiambao AB, Xu X, Pardue MT, Roveri L, McCall MA, al Ubaidi MR (2003) Loss of bipolar cells resulting from the expression of bcl-2 directed by the IRBP promoter. Exp Eye Res 77:477–483PubMedCrossRefGoogle Scholar
  22. 22.
    Joseph RM, Li T (1996) Overexpression of Bcl-2 or Bcl-XL transgenes and photoreceptor degeneration. Invest Ophthalmol Vis Sci 37:2434–2446PubMedGoogle Scholar
  23. 23.
    Strettoi E, Volpini M (2002) Retinal organization in the bcl-2-overexpressing transgenic mouse. J Comp Neurol 446:1–10PubMedCrossRefGoogle Scholar
  24. 24.
    Cenni MC, Bonfanti L, Martinou JC, Ratto GM, Strettoi E, Maffei L (1996) Long-term survival of retinal ganglion cells following optic nerve section in adult bcl-2 transgenic mice. Eur J Neurosci 8:1735–1745PubMedCrossRefGoogle Scholar
  25. 25.
    Gianfranceschi L, Fiorentini A, Maffei L (1999) Behavioural visual acuity of wild type and bcl2 transgenic mouse. Vision Res 39:569–574PubMedCrossRefGoogle Scholar
  26. 26.
    Porciatti V, Pizzorusso T, Cenni MC, Maffei L (1996) The visual response of retinal ganglion cells is not altered by optic nerve transection in transgenic mice overexpressing Bcl-2. Proc Natl Acad Sci U S A 93:14955–14959PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Cécile Péant
    • 1
  • André Dosso
    • 2
  • Lorenza Eder-Colli
    • 1
  • Florence Chiodini
    • 2
  1. 1.Department of Basic Neuroscience, Medical SchoolUniversity of GenevaGenevaSwitzerland
  2. 2.Laboratory of Cell Biology, Ophthalmology ClinicGeneva University HospitalGeneva 14Switzerland

Personalised recommendations