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Circ_0091579 Serves as a Tumor-Promoting Factor in Hepatocellular Carcinoma Through miR-1225-5p/PLCB1 Axis

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Abstract

Background

Hepatocellular carcinoma (HCC) is a dreadful threaten to human health worldwide. Many circular RNAs were reported to influence the malignant development of HCC. The aim of this study was to elucidate the role of circ_0091579 in HCC progression and the molecular fundamentation.

Methods

Expression of circ_0091579, microRNA-1225-5p (miR-1225-5p), and phospholipase C, β1 (PLCB1) was examined by quantitative reverse transcription-polymerase chain reaction or Western blotting. Cell viability, clonogenicity capacity, and apoptosis were determined via Cell Counting Kit-8 assay, colony formation assay, and flow cytometry, respectively. Transwell assay was employed to detect cell migration and invasion. Target relationship between miR-1225-5p and circ_0091579 or PLCB1 was demonstrated by dual-luciferase reporter assay. Moreover, role of circ_0091579 in vivo was assessed by Xenograft model assay.

Results

Expression of circ_0091579 and PLCB1 was increased, while miR-1225-5p expression was decreased in HCC tissues and cells. Circ_0091579 or PLCB1 depletion had inhibitory effects on HCC cell proliferation and metastasis. Circ_0091579 sponged miR-1225-5p to upregulate PLCB1 expression in HCC cells. Silencing of miR-1225-5p contributed to HCC progression, which was mitigated by PLCB1 depletion. Circ_0091579 deficiency could suppress HCC tumor growth in vivo.

Conclusion

Circ_0091579 knockdown repressed HCC progression and tumorigenesis by regulating miR-1225-5p/PLCB1 axis, affording a novel molecular basis for HCC development.

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Availability of data and materials

The analyzed data sets generated during the present study are available from the corresponding author on reasonable request.

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Acknowledgment

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Funding

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Authors and Affiliations

  1. Department of General Surgery, The Second Affiliated Hospital of Xi’an Jiaotong University, No. 157 Xiwu Road, Xincheng District, Xi’an, 710004, Shaanxi, China

    Di Zhang, Xiwu Zhang, Hongjun Zhai, Xiaoli Sun & Yiming Li

  2. Department of Gastroenterology, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, China

    Yu Zhang

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  1. Di Zhang
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  2. Yu Zhang
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  3. Xiwu Zhang
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  4. Hongjun Zhai
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  5. Xiaoli Sun
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  6. Yiming Li
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Contributions

YZ and XZ contributed to conceptualization and methodology; HZ, XS, and YL performed formal analysis and data curation; DZ, YZ, and HZ were involved in validation and investigation; DZ, YZ, XZ, and HZ performed writing—original draft preparation, and writing—review and editing; all authors approved the final manuscript.

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Correspondence to Yiming Li.

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The authors declare that they have no competing interests.

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The present study was approved by the ethical review committee of The Second Affiliated Hospital of Xi'an Jiaotong University. Written informed consent was obtained from all enrolled patients.

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Zhang, D., Zhang, Y., Zhang, X. et al. Circ_0091579 Serves as a Tumor-Promoting Factor in Hepatocellular Carcinoma Through miR-1225-5p/PLCB1 Axis. Dig Dis Sci 67, 585–597 (2022). https://doi.org/10.1007/s10620-021-06861-2

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