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Resolvin D1 Resolve Inflammation in Experimental Acute Pancreatitis by Restoring Autophagic Flux

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Abstract

Background

Acute pancreatitis (AP) is a common acute gastrointestinal disorders. Increasing evidence indicated that autophagy is involved in the development of AP. Resolvin D1 is an endogenous pro-resolving lipid mediator, which can protect mice from cerulein-induced acute pancreatitis and facilitate autophagy in macrophage, but its mechanism remians unclear.

Aims

To investigate the effect of resolvin D1 on autophagy in mouse models of cerulein-induced AP.

Methods

C57BL/6 mice were randomly divided into control group, AP group and resolvin D1 group. The models of cerulein-induced AP were constructed by intraperitoneally cerulein. Resolvin D1 group was established by intraperitoneally resolvin D1 based on AP models, simultaneously, control group received normal saline. The severity of AP, the level of inflammatory cytokines, the number of autophagic vacuoles, and the expression of autophagy-related markers were evaluated among three groups.

Results

The AP models were established successfully. Compared to control group, the number of autophagic vacuoles and expressions of autophagy-related markers including Beclin-1, p62 and LC3-II were increased in AP models, In contrast, the degree of inflammation and levels of inflammatory cytokines in AP models were reduced after resolvin D1 treatment. Moreover, resolvin D1 attenuated the number of autophagic vacuoles and expressions of autophagy-related markers.

Conclusions

Autophagic flux is impaired in cerulein-induced AP. Resolvin D1 ameliorate the severity of mice with cerulein-induced acute pancreatitis, possible attributing to its reducing impaired autophagy and restoring autophagic flux.

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Acknowledgments

The authors are grateful to Department of Pathology of the First Affiliated Hospital of Anhui Medical University for providing technical support in histological studies.

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Authors and Affiliations

  1. Department of Gastroenterology, The First Affiliated Hospital of Anhui Medical University, The Key Laboratory of Digestive Diseases of Anhui Province, 218 Jixi Road, Hefei, 230022, Anhui Province, China

    Bingbing Wang, Cui Hu, Yongyu Mei, Junjun Bao, Shaozhen Ding, Xiaochang Liu, Qiao Mei & Jianming Xu

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  1. Bingbing Wang
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  2. Cui Hu
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  3. Yongyu Mei
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  6. Xiaochang Liu
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  7. Qiao Mei
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Correspondence to Qiao Mei.

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Wang, B., Hu, C., Mei, Y. et al. Resolvin D1 Resolve Inflammation in Experimental Acute Pancreatitis by Restoring Autophagic Flux. Dig Dis Sci 63, 3359–3366 (2018). https://doi.org/10.1007/s10620-018-5191-4

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  • DOI: https://doi.org/10.1007/s10620-018-5191-4

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