Digestive Diseases and Sciences

, Volume 63, Issue 5, pp 1219–1228 | Cite as

Co-expression Network Analysis Identified COL8A1 Is Associated with the Progression and Prognosis in Human Colon Adenocarcinoma

  • Jian Shang
  • Fan Wang
  • Pengfei Chen
  • Xiaobing Wang
  • Feng Ding
  • Shi Liu
  • Qiu Zhao
Original Article


Backgrounds and Aims

Human colon adenocarcinoma is one of the major causes of tumor-induced death worldwide. A complicated gene interconnection network significantly regulates its progression and prognosis. The aim of our study was to find hub genes associated with the progression and prognosis of colon adenocarcinoma and to illustrate the underlying mechanisms.


A weighted gene co-expression network analysis was performed in our study to identify significant gene modules and hub genes associated with the TNM stage of colon adenocarcinoma (n = 441).


In the turquoise module of interest, 23 hub genes were initially selected, and 10 of them were identified as “real” hub genes with high connectivity in the protein–protein interaction network. In the terms of validation, COL8A1 had the highest correlation with clinical traits among all of the hub genes. Data obtained from the Oncomine and GEPIA databases showed a higher expression of COL8A1 in colon adenocarcinoma tissues compared with normal colon tissues. Kaplan–Meier survival curves showed that higher expression of COL8A1 resulted in a shorter overall survival time and disease-free survival time. Univariate and multivariate Cox proportional hazards analyses indicated that the COL8A1 expression was an independent prognostic factor for survival in colon adenocarcinoma patients. Finally, gene set enrichment analysis indicated that the gene sets associated with focal adhesion were significantly enriched in colon adenocarcinoma samples with COL8A1 highly expressed.


COL8A1 was identified and proved to be correlated with the progression and prognosis of human colon adenocarcinoma, probably through regulating focal adhesion-related pathways.


COL8A1 Colon adenocarcinoma Prognosis Disease progression Co-expression analysis 



We thank Dr. Fan Wang for his great contribution to the data analyses in this paper.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

10620_2018_4996_MOESM1_ESM.docx (17 kb)
Supplementary material 1 (DOCX 17 kb)
10620_2018_4996_MOESM2_ESM.tif (557 kb)
Supplementary Figure 1. Correlation between the expression levels of SPARC, COL5A2, ADAMTS2, COL3A1, COL5A1, COL6A3, COL1A2, COL8A1, COL1A1 and COL6A1 and the disease progression of colon adenocarcinoma (based on microarray data of GSE39582) (TIF 558 kb)


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Jian Shang
    • 1
    • 2
  • Fan Wang
    • 1
    • 2
  • Pengfei Chen
    • 1
    • 2
  • Xiaobing Wang
    • 1
    • 2
  • Feng Ding
    • 1
    • 2
  • Shi Liu
    • 1
    • 2
  • Qiu Zhao
    • 1
    • 2
  1. 1.Department of Gastroenterology/HepatologyZhongnan Hospital of Wuhan UniversityWuhanPeople’s Republic of China
  2. 2.The Hubei Clinical Center and Key Laboratory of Intestinal and Colorectal DiseasesWuhanPeople’s Republic of China

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