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Digestive Diseases and Sciences

, Volume 63, Issue 4, pp 910–919 | Cite as

Autophagy Strengthens Intestinal Mucosal Barrier by Attenuating Oxidative Stress in Severe Acute Pancreatitis

  • Luqiao Huang
  • Yingjian Jiang
  • Zhenqing Sun
  • Zhengyu Gao
  • Jiang Wang
  • Dianliang Zhang
Original Article

Abstract

Background

Intestinal mucosal barrier dysfunction can be caused by severe acute pancreatitis (SAP). It is normally associated with changes to mucosal autophagy and oxidative stress.

Objective

The aim of this study was to investigate the correlation between autophagy and oxidative stress on the intestinal mucosal barrier of SAP rat model.

Methods

SAP was induced by retrograde injection of sodium taurocholate (5%) into the biliopancreatic duct. Bacterial translocation (BT) was detected by 16S rDNA sequencing analysis. Morphological alterations in the pancreas and gut were determined by hematoxylin–eosin staining. Oxidative stress status was determined by measuring the level of intestinal malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GPx). Western blot, RT-PCR, and immunofluorescent staining were preformed to analyze the expression of tight junction and autophagy proteins.

Results

According to the sequencing analysis, rats in SAP group were divided into BT (+) group (n = 9) and BT (−) group (n = 8). Pancreatic and intestinal injuries in SAP group were significantly higher than sham operation group. The content of MDA was clearly elevated, and SOD as well as GPx activities were decreased in BT (+) group as compared with BT (−) group. The expression of LC3II and Beclin1 in BT (−) group was higher than that observed in BT (+). In contrast, BT (+) group had a higher level of claudin-2 and a lower level of zonula occluden-1, occludin, and claudin-1.

Conclusion

These results suggest that activated autophagy may attenuate intestinal mucosal barrier dysfunction by preventing and reducing the oxidative stress in SAP.

Keywords

Autophagy Oxidative stress Bacterial translocation Tight junction Pancreatitis 

Notes

Acknowledgments

This work was supported by the National Natural Science Foundation of China, Nos. 81270448 and 81470890.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Center of Colon and Rectum, Qingdao Municipal HospitalQingdao UniversityQingdaoChina
  2. 2.Department of General SurgeryThe Affiliated Hospital of Qingdao UniversityQingdaoChina
  3. 3.Department of Rehabilitation MedicineThe Affiliated Hospital of Qingdao UniversityQingdaoChina

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