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Digestive Diseases and Sciences

, Volume 59, Issue 2, pp 336–345 | Cite as

miR-449a Regulates Proliferation and Chemosensitivity to Cisplatin by Targeting Cyclin D1 and BCL2 in SGC7901 Cells

  • Jianghong Hu
  • Yue Fang
  • Yuan Cao
  • Rong Qin
  • Qiaoyun Chen
Original Article

Abstract

Background

Recently, several miRNAs have been determined as tumor suppressors in various cancers, such as microRNA-449a. However, the exact molecular mechanisms underlying miR-449a regulated cell proliferation and chemosensitivity in gastric cancer cells have not been well documented.

Aim

The present study was designed to test whether miR-449a mediates cell proliferation and chemosensitivity in gastric cancer cells via regulating cyclin D1 and BCL2.

Methods

In vitro, the ability of cell proliferation and cell viability were measured by MTT assay; cell cycle and cell apoptosis was detected by FCM. qRT-PCR was used to measure the expression of miR-449a. Western blot and real-time PCR assays were used to detect the expression of cyclin D1 and BCL2 in gastric cancer cell line SGC7901.

Results

miR-449a expression was downregulated in gastric cancer cell line SGC7901 and human gastric cancer tissues, compared to the gastric epithelial cell line GES-1 and matched non-tumor associated tissues. Upregulation of miR-449a reduced the proliferation of SGC7901 cells. Ectopic expression of miR-449a decreased the percentage of S phase cells, increased the percentage of G1/G0 phase cells and increased the apoptosis induced by cisplatin. Moreover, miR-449a inhibited SGC7901 cells proliferation and enhanced cisplatin chemosensitivity by downregulating expression of BCL2 and cyclin D1, respectively, via directly targeting the 3′-untranslated regions of BCL2 and cyclin D1 mRNA.

Conclusions

This is the first report to provide evidence that miR-449a could modulate cell cycle and apoptosis through regulating cyclin D1 and BCL2 expression in SGC7901 cells.

Keywords

miR-449a BCL2 protein Cyclin D1 protein Gastric cancer 

Notes

Conflict of interest

None.

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Jianghong Hu
    • 1
  • Yue Fang
    • 2
  • Yuan Cao
    • 2
  • Rong Qin
    • 3
  • Qiaoyun Chen
    • 4
  1. 1.Department of DigestionThe Danyang People’s HospitalDanyangPeople’s Republic of China
  2. 2.Department of Central Laboratory, The Fourth Affiliated People’s HospitalJiangsu UniversityZhenjiangPeople’s Republic of China
  3. 3.Department of Oncology, The Affiliated of People’s HospitalJiangsu UniversityZhenjiangPeople’s Republic of China
  4. 4.Department of Central Laboratory, The Affiliated People’s HospitalJiangsu UniversityZhenjiangPeople’s Republic of China

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