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Digestive Diseases and Sciences

, Volume 58, Issue 2, pp 354–362 | Cite as

Attenuation of Acetic Acid-Induced Gastric Ulcer Formation in Rats by Glucosylceramide Synthase Inhibitors

  • Manabu Nakashita
  • Hidekazu Suzuki
  • Soichiro Miura
  • Takao Taki
  • Keita Uehara
  • Tohru Mizushima
  • Hiroshi Nagata
  • Toshifumi Hibi
Original Article

Abstract

Introduction

Ceramide has been suggested to play a role in apoptosis during gastric ulcerogenesis. The present study is designed to investigate whether accumulated ceramide could serve as the effector molecules of ulcer formation in a rat model of acetic acid-induced gastric ulcer.

Methods

The effect of fumonisin B1, an inhibitor of ceramide synthase, and of d,l,-threo-1-phenyl-2-hexadecanoylamino-3-morpholino-1-propanol (PPMP) and N-butyldeoxynojirimycin (NB-DNJ), both inhibitors of glucosylceramide synthase, on the accumulation of ceramide and formation of gastric ulcer were examined in the rat model of acetic acid-induced gastric ulcer.

Results

Fumonisin B1 attenuated acetic acid-induced gastric ulcer formation, associated with a decrease in the number of apoptotic cells. Our results showed that it is neither the C18- nor the C24-ceramide itself, but the respective metabolites that were ulcerogenic, because PPMP and NB-DNJ attenuated gastric mucosal apoptosis and the consequent mucosal damage in spite of their reducing the degradation of ceramide.

Conclusion

The ceramide pathway, in particular, the metabolites of ceramide, significantly contributes to acetic acid-induced gastric damage, possibly via enhancing apoptosis. On the other hand, PPMP and NB-DNJ treatment attenuated gastric mucosal apoptosis and ulcer formation despite increasing the ceramide accumulation, suggesting that it was not the ceramides themselves, but their metabolites that contributed to the ulcer formation in the acetic acid-induced gastric ulcer model.

Keywords

Ceramide Glucosylceramide inhibitor Gastric ulcer Acetic acid Apoptosis 

Notes

Acknowledgments

This study was supported by a Grant-in-Aid for Scientific Research (B) from the Japan Society for the Promotion of Science (22300169, to H.S.), Grant-in-Aid for challenging Exploratory Research (24659103, to H.S.), a grant from the National Defense Medical College (to S.M.), a Research Fund of Mitsukoshi Health and Welfare Foundation (to H.S.), and a grant from the Smoking Research Foundation (to H.S.).

Conflict of interest

None.

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Manabu Nakashita
    • 1
    • 5
  • Hidekazu Suzuki
    • 1
  • Soichiro Miura
    • 2
  • Takao Taki
    • 3
  • Keita Uehara
    • 1
  • Tohru Mizushima
    • 4
  • Hiroshi Nagata
    • 1
    • 5
  • Toshifumi Hibi
    • 1
  1. 1.Division of Gastroentrology and Hepatology, Department of Internal Medicine, School of MedicineKeio UniversityShinjuku-ku, TokyoJapan
  2. 2.Second Department of Internal MedicineNational Defense Medical CollegeSaitamaJapan
  3. 3.Molecular Medical Science InstituteOtsuka Pharmaceutical Co. LtdTokushimaJapan
  4. 4.Faculty of PharmacyKeio UniversityTokyoJapan
  5. 5.Department of Internal MedicineKeiyu HospitalYokohamaJapan

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