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Lactobacillus plantarum Prevents Bacterial Translocation in Rats Following Ischemia and Reperfusion Injury

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Abstract

Background

Bacterial translocation is considered a major cause of initiation and development of systemic sepsis and multiple organ dysfunction in clinic. The aim of this study was to determine the ability of a defined Lactobacillus plantarum to prevent ischemia/reperfusion (I/R) induced intestinal infection.

Methods

Female Sprague-Dawley rats were randomly allocated into three groups: (1) controls (sham-operated, no treatment), (2) ischemia/reperfusion and (3) ischemia/reperfusion and Lactobacillus plantarum treatment. Lactobacillus plantarum L2 was administered daily intragastrically 14 days prior to induction of I/R. Rats were then sacrificed, and tissue and blood samples were cultured to determine bacterial translocation. Cytokines in plasma were detected by ELISA. Ileal segments were removed for morphological examination.

Results

Intestinal I/R induced excess pro-inflammatory cytokine secretion and barrier dysfunction (increased epithelial cell apoptosis, cecal flora dysbiosis, disruption of mucosa and multiple erosions) in the intestine, associated with increased bacterial translocation to extraintestinal sites. Approximately 87.5% of rats exposed to I/R had bacterial translocation while there was no bacterial translocation in controls. However, pretreatment of animals with Lactobacillus plantarum completely prevented I/R induced bacterial translocation, reduced pro-inflammatory cytokine release, and intestinal epithelial cell apoptosis, resulting in recovered microflora and mucosal integrity.

Conclusions

These findings indicate that Lactobacillus plantarum L2 can prevent I/R-induced bacterial translocation and intestinal barrier dysfunction and, thereby, exert beneficial effects in the intestinal tract.

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Acknowledgments

The present work was financially supported by the National Natural Science Foundation of China (Grants 30801090 and 30830098).

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Authors and Affiliations

  1. Department of Surgery, Research Institute of General Surgery, Jin Ling Hospital, Nanjing, Jiangsu, 210002, People’s Republic of China

    Bin Wang, Qian Huang, Wei Zhang, Ning Li & Jieshou Li

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  1. Bin Wang
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  2. Qian Huang
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  3. Wei Zhang
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  4. Ning Li
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  5. Jieshou Li
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Correspondence to Jieshou Li.

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Wang, B., Huang, Q., Zhang, W. et al. Lactobacillus plantarum Prevents Bacterial Translocation in Rats Following Ischemia and Reperfusion Injury. Dig Dis Sci 56, 3187–3194 (2011). https://doi.org/10.1007/s10620-011-1747-2

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  • DOI: https://doi.org/10.1007/s10620-011-1747-2

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