Skip to main content

Advertisement

SpringerLink
Log in

Serotonin Transporter and G Protein Beta 3 Subunit Gene Polymorphisms in Greeks with Irritable Bowel Syndrome

  • Original Article
  • Published:
Digestive Diseases and Sciences Aims and scope Submit manuscript

Abstract

Background

Polymorphisms in the serotonin transporter (SERT) and G protein β3 subunit (GNB3) genes might contribute to the pathophysiology of irritable bowel syndrome (IBS). Association studies of SERT and GNB3 polymorphisms and IBS have shown diverse results among different populations, which might be due to subject composition differences.

Aims

The aim of the study was to assess the potential association between SERT and GNB3 polymorphisms and IBS in Greeks.

Methods

A total of 124 patients with IBS diagnosed according to the Rome III criteria and 238 healthy individuals were included in the study. SERT and GNB3 gene polymorphisms were genotyped using polymerase chain reaction-based methods.

Results

It was shown that the frequencies of the SS genotype and S allele of the serotonin transporter polymorphism were significantly associated with IBS (P = 0.0314 and P = 0.019, respectively). TT genotype and T allele frequencies of G protein β3 subunit showed also significant difference between the IBS patients and healthy controls IBS (P = 0.0163 and P = 0.0001, respectively). None of the clinical symptoms analyzed was significantly associated with the polymorphisms tested.

Conclusions

The results suggest that SERT and GNB3 gene polymorphisms might be associated with irritable bowel syndrome predisposition in Greeks.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  1. Longstreth GF, Thompson WG, Chey WD, Houghton LA, Mearin F, Spiller RC. Functional bowel disorders. Gastroenterology. 2006;130:1480–1491.

    Article  PubMed  Google Scholar 

  2. Drossman DA, Dumitrascu DL. Rome III: New standard for functional gastrointestinal disorders. J Gastrointestin Liver Dis. 2006;15:237–241.

    PubMed  Google Scholar 

  3. Buéno L, Fioramonti J, Garcia-Villar R. Pathobiology of visceral pain: molecular mechanisms and therapeutic implications. III. Visceral afferent pathways: a source of new therapeutic targets for abdominal pain. Am J Physiol Gastrointest Liver Physiol. 2000;278:G670–G676.

    PubMed  Google Scholar 

  4. Manabe N, Tanaka T, Hata J, Kusunoki H, Haruma K. Pathophysiology underlying irritable bowel syndrome—from the viewpoint of dysfunction of autonomic nervous system activity. J Smooth Muscle Res. 2009;45:15–23.

    Article  PubMed  Google Scholar 

  5. Sikander A, Rana SV, Prasad KK. Role of serotonin in gastrointestinal motility and irritable bowel syndrome. Clin Chim Acta. 2009;403:47–55.

    Article  PubMed  CAS  Google Scholar 

  6. Crowell MD. Role of serotonin in the pathophysiology of the irritable bowel syndrome. Br J Pharmacol. 2004;141:1285–1293.

    Article  PubMed  CAS  Google Scholar 

  7. Locke GR 3rd, Zinsmeister AR, Talley NJ, Fett SL, Melton LJ 3rd. Familial association in adults with functional gastrointestinal disorders. Mayo Clin Proc. 2000;75:907–912.

    Article  PubMed  Google Scholar 

  8. Lembo A, Zaman M, Jones M, Talley NJ. Influence of genetics on irritable bowel syndrome, gastro-oesophageal reflux and dyspepsia: a twin study. Aliment Pharmacol Ther. 2007;25:1343–1350.

    Article  PubMed  CAS  Google Scholar 

  9. Saito YA, Locke GR 3rd, Zimmerman JM, et al. A genetic association study of 5-HTT LPR and GNbeta3 C825T polymorphisms with irritable bowel syndrome. Neurogastroenterol Motil. 2007;19:465–470.

    Article  PubMed  CAS  Google Scholar 

  10. Camilleri M, Atanasova E, Carlson PJ, et al. Serotonin-transporter polymorphism pharmacogenetics in diarrhea-predominant irritable bowel syndrome. Gastroenterology. 2002;123:425–432.

    Article  PubMed  CAS  Google Scholar 

  11. Jarrett ME, Kohen R, Cain KC, et al. Relationship of SERT polymorphisms to depressive and anxiety symptoms in irritable bowel syndrome. Biol Res Nurs. 2007;9:161–169.

    Article  PubMed  CAS  Google Scholar 

  12. Yeo A, Boyd P, Lumsden S, et al. Association between a functional polymorphism in the serotonin transporter gene and diarrhoea predominant irritable bowel syndrome in women. Gut. 2004;53:1452–1458.

    Article  PubMed  CAS  Google Scholar 

  13. Pata C, Erdal ME, Derici E, Yazar A, Kanik A, Ulu O. Serotonin transporter gene polymorphism in irritable bowel syndrome. Am J Gastroenterol. 2002;97:1780–1784.

    Article  PubMed  CAS  Google Scholar 

  14. Lee DY, Park H, Kim WH, Lee SI, Seo YJ, Choi YC. Serotonin transporter gene polymorphism in healthy adults and patients with irritable bowel syndrome. Korean J Gastroenterol. 2004;43:18–22.

    PubMed  Google Scholar 

  15. Niesler B, Kapeller J, Fell C, et al. 5-HTTLPR and STin2 polymorphisms in the serotonin transporter gene and irritable bowel syndrome: effect of bowel habit and sex. Eur J Gastroenterol Hepatol. 2010;22:856–861.

    Article  PubMed  CAS  Google Scholar 

  16. Zill P, Barghai TC, Zwanzger P, et al. Evidence for an association between a G-protein beta3-gene variant with depression and response to antidepressant treatment. Neuroreport. 2000;11:1893–1897.

    Article  PubMed  CAS  Google Scholar 

  17. Virchow S, Ansorge N, Rübben H, Siffert G, Siffert W. Enhanced fMLP-stimulated chemotaxis in human neutrophils from individuals carrying the G protein beta3 subunit 825 T-allele. FEBS Lett. 1998;436:155–158.

    Article  PubMed  CAS  Google Scholar 

  18. Siffert W. G-protein beta3 subunit 825T allele and hypertension. Curr Hypertens Rep. 2003;5:47–53.

    Article  PubMed  Google Scholar 

  19. Lee HJ, Lee SY, Choi JE, et al. G protein beta3 subunit, interleukin-10, and tumor necrosis factor-alpha gene polymorphisms in Koreans with irritable bowel syndrome. Neurogastroenterol Motil. 2010;22:758–763.

    Article  PubMed  CAS  Google Scholar 

  20. Andresen V, Camilleri M, Kim HJ, et al. Is there an association between GNbeta3–C825T genotype and lower functional gastrointestinal disorders? Gastroenterology. 2006;130:1985–1994.

    Article  PubMed  CAS  Google Scholar 

  21. Holtmann G, Siffert W, Haag S, et al. G-protein beta 3 subunit 825 CC genotype is associated with unexplained (functional) dyspepsia. Gastroenterology. 2004;126:971–979.

    Article  PubMed  CAS  Google Scholar 

  22. Coffin B. Diagnosis of irritable bowel syndrome. Gastroenterol Clin Biol. 2009;33:S9–S16.

    Article  PubMed  Google Scholar 

  23. Park JM, Choi MG, Park JA, et al. Serotonin transporter gene polymorphism and irritable bowel syndrome. Neurogastroenterol Motil. 2006;18:995–1000.

    Article  PubMed  CAS  Google Scholar 

  24. Lesch KP, Bengel D, Heils A, et al. Association of anxiety-related traits with a polymorphism in the serotonin transporter gene regulatory region. Science. 1996;274:1527–1531.

    Article  PubMed  CAS  Google Scholar 

  25. Collier DA, Stöber G, Li T, et al. A novel functional polymorphism within the promoter of the serotonin transporter gene: possible role in susceptibility to affective disorders. Mol Psychiatry. 1996;1:453–460.

    PubMed  CAS  Google Scholar 

  26. Sikander A, Rana SV, Sinha SK, et al. Serotonin transporter promoter variant: analysis in Indian IBS patients and control population. J Clin Gastroenterol. 2009;43:957–961.

    Article  PubMed  CAS  Google Scholar 

  27. de Vries DR, ter Linde JJ, van Herwaarden MA, Smout AJ, Samsom M. Gastroesophageal reflux disease is associated with the C825T polymorphism in the G-protein beta3 subunit gene (GNB3). Am J Gastroenterol. 2009;104:281–285.

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. 2nd Department of Gastroenterology, Evangelismos General Hospital, Athens, Greece

    T. Markoutsaki & D. G. Karamanolis

  2. Department of Basic Sciences, Laboratory of Biology, School of Medicine, University of Athens, Michalakopoulou 176, 11527, Athens, Greece

    T. Karantanos, M. Gazouli & N. P. Anagnou

  3. Laboratory of Cell and Gene Therapy, Center of Basic Research II, Foundation for Biomedical Research of the Academy of Athens (IIBEAA), Athens, Greece

    N. P. Anagnou

  4. Gastroenterology Division, 1st Propaedeutic Department of Internal Medicine, School of Medicine, Laikon Hospital, University of Athens, Athens, Greece

    S. D. Ladas

Authors
  1. T. Markoutsaki
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. T. Karantanos
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Gazouli
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. N. P. Anagnou
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. S. D. Ladas
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. D. G. Karamanolis
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to M. Gazouli.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Markoutsaki, T., Karantanos, T., Gazouli, M. et al. Serotonin Transporter and G Protein Beta 3 Subunit Gene Polymorphisms in Greeks with Irritable Bowel Syndrome. Dig Dis Sci 56, 3276–3280 (2011). https://doi.org/10.1007/s10620-011-1726-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10620-011-1726-7

Keywords

Search

Navigation