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Digestive Diseases and Sciences

, Volume 56, Issue 10, pp 2810–2817 | Cite as

The Protective Effect of the Recombinant 53-kDa Protein of Trichinella spiralis on Experimental Colitis in Mice

  • Linlin Du
  • Hao Tang
  • Zhongfu Ma
  • Jia Xu
  • Wenchao Gao
  • Jiajia Chen
  • Wenjia Gan
  • Zhaoping Zhang
  • Xinbing Yu
  • Xingwang Zhou
  • Xuchu Hu
Original Article

Abstract

Background

Helminth infection has been proven to reduce the severity of experimental inflammatory bowel disease (IBD). The excretory-secretory proteins of helminths play an important role in the process of immunomodulation.

Aims

In the present study, we aimed to investigate the protective potential of recombinant Trichinella spiralis (TS) 53-kDa protein (rTsP53), a component of excretory-secretory proteins, on experimental colitis in mice.

Methods

BALB/c mice were treated subcutaneously with 50 μg rTsP53 three times at an interval of 5 days. Colitis was induced by intrarectal administration of 5 mg trinitrobenzene sulfonic acid (TNBS). Disease activities and macroscopic and microscopic scores were evaluated. To determine immune response provoked by rTsP53, we measured specific IgG1 and IgG2a values against rTsP53 in sera of mice. We also detected cytokine profiles as well as the markers of alternatively activated macrophages (M2) in mice.

Results

RTsP53 ameliorated significantly the disease activity index (DAI) as well as the macroscopic and microscopic scores. IgG1 but not IgG2a was the predominant specific antibody detected in the sera of immunized mice, indicating the potential of stimulating T-helper (Th) 2 bias response by rTsP3. Pre-treatment with rTsP53 decreased serum Th1 cytokines (TNF-a, IFN-γ) and elevated serum levels of serum Th2 cytokines (IL-4, IL-13); it also decreased colonic Th1 cytokines (TNF-α, IL-6) and colonic regulatory cytokines (IL-10, TGF-β1). RTsP53 increased colonic M2 markers, arginase-1 (Arg-1), and found in inflammatory zone 1 (FIZZ1), compared to mice without rTsP53 pretreatment.

Conclusions

RTsP53 is a potential protective agent for IBD.

Keywords

Inflammatory bowel disease 53-kDa protein Trichinella spiralis Alternatively activated macrophages Th2 

Notes

Acknowledgments

This work was supported by the China National Great Basic Research Program (the 973 program, No. 2010CB530003) and the China National Natural Science Foundation (No. 30771887).

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Linlin Du
    • 1
  • Hao Tang
    • 1
  • Zhongfu Ma
    • 1
  • Jia Xu
    • 1
  • Wenchao Gao
    • 2
  • Jiajia Chen
    • 2
  • Wenjia Gan
    • 2
  • Zhaoping Zhang
    • 2
  • Xinbing Yu
    • 2
  • Xingwang Zhou
    • 3
  • Xuchu Hu
    • 2
    • 4
  1. 1.Department of General Internal Medicine, The First Affiliated HospitalSun Yat-sen UniversityGuangzhouChina
  2. 2.Department of Parasitology, Zhongshan School of MedicineSun Yat-sen UniversityGuangzhouChina
  3. 3.Department of Biochemistry, Zhongshan School of MedicineSun Yat-sen UniversityGuangzhouChina
  4. 4.GuangzhouChina

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