5-Aminosalicylic Acid (5-ASA) Can Reduce Levels of Oxidative DNA Damage in Cells of Colonic Mucosa With and Without Fecal Stream
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No studies have evaluated the effectiveness of 5-ASA against oxidative DNA damage in experimental models of diversion colitis.
To evaluate the effects of 5-ASA against oxidative DNA damage in an experimental model of diversion colitis.
Twenty-six Wistar rats were divided into two groups corresponding to sacrifice at 2 or 4 weeks after fecal diversion of the left colon by means of proximal colostomy and distal mucosa fistula. Each group was divided into two subgroups according to intervention in excluded colon performed with 0.9% saline solution or 5-ASA. Level of oxidative DNA damage was determined by comet assay in cells obtained from segments with and without fecal stream before and after H2O2 challenge. For statistical analysis, was used one-way analysis of variance (ANOVA), adopting a 5% significance level (P < 0.05).
Levels of DNA damage were always higher in colon segments without fecal stream, regardless of the intervention solution employed. DNA damage in colon segments with and without fecal stream in animals irrigated with 5-ASA was lower when compared with those treated with saline solution, regardless of time of irrigation. These levels remained lower after intervention with 5-ASA, even after H2O2 challenge.
Enema with 5-ASA reduces oxidative DNA damage in epithelial cells of colon segments without fecal stream, even after H2O2 challenge, confirming the effects of 5-ASA against DNA damage by oxygen free radicals.
KeywordsColitis Mesalamine 5-Aminosalicylic acid Fatty acids Volatile Butyrates Rats
C.A.R.M. thanks the Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP) for financial support. Process. N°. 2006/02306-6.
Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP), São Paulo, Brazil.
- 5.Haas PA, Haas GP. A critical evaluation of the Hartmann’s procedure. Am Sur. 1988;54:380–385.Google Scholar
- 10.Habr-Gama A, Teixeira MG, Vieira MJF, et al. Operaçäo de Hartmann e suas conseqüências. Rev Bras Coloproctol. 1997;17:5–10.Google Scholar
- 12.Curi A, Mascarenhas JCS, Moreira Junior H, et al. Morbimortalidade associada à reconstrução do trânsito intestinal—Análise de 67 casos. Rev Bras Coloproct. 2002;22:88–97.Google Scholar
- 23.Nassri CGG, Nassri AB, Favero E, et al. Influência da irrigação de soluções nutricionais no colo excluso de trânsito intestinal. Estudo experimental em ratos. Rev Bras Coloproct. 2008;28:306–314.Google Scholar
- 38.Martinez CA, Bartocci PC, do Carmo CV, Pereira JA, Miranda DD, Ribeiro ML. The effects of oxidative DNA damage and mutations in the p53 protein on cells of the colonic mucosa with and without the fecal stream: an experimental study in rats. Scand J Gastroenterol. 2010;45:714–724.PubMedCrossRefGoogle Scholar
- 64.Wentzel JF, Gouws C, Huysamen C, DyK EV, Koekemoer G, Pretorius PJ. Assessing the DNA methylation status of single cells with comet assay. Anal Biochem. 2010 Feb 13 [Epub ahead of print].Google Scholar
- 65.Naganuma M, Iwao Y, Ogata H, et al. Measurement of colonic mucosal concentrations of 5-aminosalicylic acid is useful for estimating its therapeutic efficacy in distal ulcerative colitis: comparison of orally administered mesalamine and sulfasalazine. Inflamm Bowel Dis. 2001;7:221–225.PubMedCrossRefGoogle Scholar