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Digestive Diseases and Sciences

, Volume 54, Issue 10, pp 2198–2206 | Cite as

Down-regulation of Protein and mRNA Expression of IL-8 and ICAM-1 in Colon Tissue of Ulcerative Colitis Patients by Partition-Herb Moxibustion

  • En-Hua Zhou
  • Hui-Rong Liu
  • Huan-Gan Wu
  • Zheng Shi
  • Wei Zhang
  • Yi Zhu
  • Da-Ren Shi
  • Shuang Zhou
Original Article

Abstract

Our previous studies have demonstrated the efficacy of partition-herb moxibustion on ulcerative colitis (UC) rats. However, the mechanism of actions of partition-herb moxibustion is still unclear. Most Chinese acupuncturists believe that partition-herb improves the effect of moxibustion therapy. However, this argument lacks confirming experimental and clinical evidence. So, whether partition-herb does play a role in the mechanism of actions of partition-herb moxibustion was reported in this paper. A total of 123 patients were randomly divided into the partition-herb moxibustion group and the control group (partition-bran moxibustion group). Fourteen patients dropped out of the study. Finally, 109 patients finished the intervention. Sixty-one patients with UC received partition-herb moxibustion and 48 patients with UC received partition-bran moxibustion for 72 treatments, one treatment per day, every 12 treatments with an interval of 3 days. The expressions of interleukin-8 (IL-8) and intercellular adhesion molecule-1 (ICAM-1) in the colonic mucosa tissue in mild patients with UC were assayed by immunohistochemistry and RT-PCR. Histology of the colon also was observed at entry and after 72 treatments. Clinical therapeutic effect in partition-herb moxibustion was significantly better than those in partition-bran moxibustion (< 0.05). Protein and mRNA expression of IL-8, ICAM-1 in the patients was inhibited by both partition-herb moxibustion and partition-bran moxibustion, of those the inhibition in the partition-herb moxibustion group was more obvious (< 0.01). Partition-herb moxibustion was shown to significantly improve the histology of the colon, and partition-herb was shown to improve the therapeutic effect of moxibustion on treatment of UC patients.

Keywords

Ulcerative colitis Moxibustion Partition-herb moxibustion Gene expression Immunohistochemistry RT-PCR 

Abbreviations

ICAM-1

Intercellular adhesion molecule-1

IL-8

Interleukin-8

UC

Ulcerative colitis

Notes

Acknowledgments

This work was supported by the National Basic Research Program of China (973 Program) (2009CB522900), Shanghai Leading Academic Discipline Project (S30304), and the National Science Foundation of China (90709029).

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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • En-Hua Zhou
    • 1
  • Hui-Rong Liu
    • 1
  • Huan-Gan Wu
    • 1
  • Zheng Shi
    • 1
  • Wei Zhang
    • 2
  • Yi Zhu
    • 1
  • Da-Ren Shi
    • 3
  • Shuang Zhou
    • 1
  1. 1.Shanghai Institute of Acupuncture-Moxibustion and MeridiansShanghaiPeople’s Republic of China
  2. 2.Acupuncture DepartmentXiamen Hosptial of T.C.MXiamenPeople’s Republic of China
  3. 3.Cancer HospitalFudan UniversityShanghaiPeople’s Republic of China

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