Digestive Diseases and Sciences

, Volume 54, Issue 3, pp 530–537 | Cite as

Genetic Recombinant Expression and Characterization of Human Augmenter of Liver Regeneration

  • Chun-Fang Gao
  • Fei Guo Zhou
  • Hao Wang
  • Ying-Feng Huang
  • Qiang Ji
  • Jie Chen
Original Article


Aims To establish a highly effective prokaryotic recombinant expression system for human augmenter of liver regeneration (hALR) and to characterize the recombinant hALR both in vitro and in vivo. Methods ALR cDNA was synthesized and inserted into expression vector pET28a+, the recombinant plasmid was transformed into BL21, and expression of hALR was induced by IPTG. Recombinant hALR (rhALR) was purified by sequential detergent wash, enterokinase (EK) digestion, gel-filtration, and chelating chromatography. The rhALR was identified by SDS-PAGE, immunoblotting, MALDI-TOF-MS, and N-terminal sequencer. Cell proliferative effect of rhALR on human hepatocytes was analyzed by MTT. The protective effect of rhALR on liver function was observed on CCl4-induced intoxicated mice. Results Recombinant expression plasmid of ALR [pET28(a+)-hALR] was confirmed by restriction enzyme digestion and DNA sequencing. The expressed rhALR constituted 30% of total bacterial protein. Molecular weight was 15,029 for monomer and 30,136 for dimer by mass determination. N-terminal was M-R-T-Q-Q, exactly the same as anticipated for hALR. The purified protein migrating at about 15 KD showed excellent antigenicity in immunoblotting. The rhALR also showed a strong stimulative effect on hepatocyte proliferation. ALT and AST levels, liver histological structure, as well as the survival rate of CCl4-intoxicated mice were significantly improved when rALR was administrated at 40 μg/kg or 200 μg/kg. Conclusions The rhALR is successfully expressed highly effectively with anticipated MW, N-terminal, and antigenicity. It could play an important role in relieving acute hepatic injury and hepatic failure by promoting hepatic cell proliferation and improving liver function in CCl4-intoxicated mice.


Gene Augmenter of liver regeneration (ALR) Recombinant Hepatocytes Liver function 



Augmenter of liver regeneration




Plasmid for expression by T7 RNA polymerase


Molecular weight



We are especially grateful for the technical support of Dr. Tong-Hai Dou from Fu-Dan University regarding protein purification.


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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Chun-Fang Gao
    • 1
  • Fei Guo Zhou
    • 2
  • Hao Wang
    • 3
  • Ying-Feng Huang
    • 4
  • Qiang Ji
    • 1
  • Jie Chen
    • 4
  1. 1.Department of Laboratory MedicineEastern Hepatobiliary Hospital, Second Military Medical UniversityShanghaiChina
  2. 2.Department of Hepatic SurgeryEastern Hepatobiliary Hospital, Second Military Medical UniversityShanghaiChina
  3. 3.Department of Laboratory MedicineChangzheng Hospital, Second Military Medical UniversityShanghaiChina
  4. 4.Liver Fibrosis Research CenterChangzheng Hospital, Second Military Medical UniversityShanghaiChina

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