Increased 5-Hydroxytryptamine Mediates Post-Inflammatory Visceral Hypersensitivity via the 5-Hydroxytryptamine 3 Receptor in Rats

  • Yun-Dong Choi
  • Tae-Sik Sung
  • Hyun-Ju Kim
  • Jun-Ho La
  • Tae-Wan Kim
  • Il-Suk Yang
Original Paper


Visceral hypersensitivity often develops after intestinal inflammation, but the pathogenic mechanism has not been clearly elucidated. We investigated whether this post-inflammatory visceral hypersensitivity is mediated by 5-hydroxytryptamine through activation of the 5-hydroxytryptamine 3 receptor. In male Sprague–Dawley rats recovered from acetic acid-induced colitis, we monitored visceral nociceptive response by scoring the abdominal withdrawal reflex and simultaneously measuring the changes in arterial pulse rate. Seven days after induction of colitis, 52% of the rats showed an increased abdominal withdrawal reflex score and arterial pulse rate changes to colorectal distension, indicating that they had post-inflammatory visceral hypersensitivity. The 5-hydroxytryptamine 3 receptor antagonists, alosetron (20 mg/kg, p.o.) and granisetron (10 μg/kg, s.c.), inhibited post-inflammatory visceral hypersensitivity. Administration of a 5-hydroxytryptamine precursor, 5-hydroxytryptophan; 10 mg/kg, s.c.), induced visceral hypersensitivity in naïve rats, which was antagonized by granisetron. Increase in 5-hydroxytryptamine immunoreactive cells in colonic mucosal layer was found both in the rats with post-inflammatory visceral hypersensitivity and in the 5-hydroxytryptophan-treated rats. These results suggest that increased 5-hydroxytryptamine in colonic mucosa mediates post-inflammatory visceral hypersensitivity through activation of the 5-hydroxytryptamine 3 receptor.


5-Hydroxytryptamine Colorectal distension Post-Inflammatory visceral hypersensitivity Intestinal inflammation 


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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Yun-Dong Choi
    • 1
  • Tae-Sik Sung
    • 1
  • Hyun-Ju Kim
    • 1
  • Jun-Ho La
    • 1
  • Tae-Wan Kim
    • 2
  • Il-Suk Yang
    • 1
  1. 1.Department of Physiology, College of Veterinary MedicineSeoul National UniversitySeoulRepublic of Korea
  2. 2.Department of Physiology, College of Veterinary MedicineKyungpuk National UniversityDaeguRepublic of Korea

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