Digestive Diseases and Sciences

, Volume 53, Issue 3, pp 723–729 | Cite as

An Anti-adenoma Antibody, Adnab-9, May Reflect the Risk for Neoplastic Progression in Familial Hamartomatous Polyposis Syndromes

  • Martin Tobi
  • Michael Kam
  • Nadeem Ullah
  • Kashif Qureshi
  • Violeta Yordanova
  • James Hatfield
  • Suzanne E. G. Fligiel
  • Paula Sochacki
  • Thomas McGarrity
  • Carolyn Cole
  • Michael Lawson
  • Russell Jacoby
Original Paper


Patients with the hamartomatous polyposis Peutz-Jeghers and familial juvenile polyposis syndromes are predisposed to colorectal cancer but lack early genetic alterations found in adenomatous premalignant lesions. We studied hamartomatous polyps for the expression of an early preneoplastic colorectal neoplasia risk marker also found in familial adenomatous polyposis patients. Retrospective, genetic, and hospital archival tissue immunohistochemistry using Adnab-9, a premalignant marker often found in Paneth-like cells (PCs), was performed on sections of polyps from eight patients with Peutz-Jeghers syndrome, eight patients with familial juvenile polyposis, and 36 hyperplastic polyp control sections. Anti-α-defensin 5 (AD5), a universal PC marker, was also used to label a subgroup of sections. Hamartomatous polyposis patients also underwent specific genetic analysis. Eighty-nine percent of Peutz-Jeghers syndrome polyps labeled with Adnab-9 compared with 63% for AD5; 88% of familial juvenile polyposis sections also labeled with Adnab-9. Of the 36 hyperplastic polyp sections, only four (11%) labeled with Adnab-9 and one (3%) with AD5. Adnab-9 labeling of PCs in the epithelial elements of hamartomatous colonic lesions of hereditary hamartomatous syndrome patients reflects the predisposition to colorectal cancer, further justifying early intervention strategies.


Adnab-9 Peutz-Jeghers syndrome Familial juvenile polyposis Anti-α-defensin 5 Paneth-like cells 



Adenomatous polyposis coli


Colorectal cancer




Hereditary familial juvenile polyposis syndrome


Inflammatory bowel disease


Optical densitometry


Peutz-Jeghers syndrome



The authors would like to express their gratitude to Susan Katz, BA, for assistance with specimen collection. The work was supported in part by a Department of Veterans Affairs (VAMC) grant awarded to Dr. Martin Tobi, a Kaiser Permanente Research Foundation grant to Dr. Michael Lawson, and a VAMC Merit Review Grant to Dr. Russell Jacoby. This paper is dedicated to the memory of Irwin Stein.


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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Martin Tobi
    • 1
    • 2
  • Michael Kam
    • 1
  • Nadeem Ullah
    • 1
  • Kashif Qureshi
    • 1
  • Violeta Yordanova
    • 1
  • James Hatfield
    • 1
  • Suzanne E. G. Fligiel
    • 1
  • Paula Sochacki
    • 1
  • Thomas McGarrity
    • 3
  • Carolyn Cole
    • 4
  • Michael Lawson
    • 5
  • Russell Jacoby
    • 4
  1. 1.John D. Dingell VAMC Departments of Medicine, Gastroenterology and PathologyWayne State University School of MedicineDetroitUSA
  2. 2.Section Gastroenterology MED III/GIPhiladelphia VAMCPhiladelphiaUSA
  3. 3.Department of Medicine, The Milton S. Hershey Medical CenterThe Pennsylvania State University College of MedicineHersheyUSA
  4. 4.Wm. S. Middelton Memorial VAMCUniversity Wisconsin Comprehensive Cancer CenterMadisonUSA
  5. 5.Kaiser Permanente Medical GroupSacramentoUSA

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