Digestive Diseases and Sciences

, Volume 53, Issue 2, pp 363–369 | Cite as

Helicobacter Pylori Infection Induces Candidate Stem Cell Marker Musashi-1 in the Human Gastric Epithelium

  • Hiroaki Murata
  • Shingo Tsuji
  • Masahiko Tsujii
  • Toru Nakamura
  • Hai Ying Fu
  • Hiroshi Eguchi
  • Kayoko Asahi
  • Hideyuki Okano
  • Sunao Kawano
  • Norio Hayashi
Original Paper


Musashi-1 (Msi-1), a mammalian neural RNA-binding protein, has been found to play important roles in the maintenance of stem cell states and differentiation in neural stem cells and mouse intestinal cells. We explored Msi-1 expression and its potential implications in the human stomach. Reverse transcription-PCR revealed that Msi-1 levels were significantly higher in the corpus than in antrum in Helicobacter pylori (Hp)-infected patients (n = 49) (P < 0.00001) in paired biopsy samples, whereas they were low and comparable at these two sites in Hp-negative patients (n = 31). Msi-1 levels were significantly higher in the Hp-infected corpus (n = 107) than in the Hp-negative corpus (n = 69) (P < 0.00000001). Immunohistochemistry and in situ hybridization demonstrated that Msi-1 was expressed at the base and neck/isthmus region of the fundic glands and partly co-expressed in Ki-67-positive cells in the corpus and antrum. Msi-1 levels correlated with Hp density (P < 0.05). Based on these results, we conclude that Hp infection strongly induces Msi-1 in the corpus. Given its expression in dividing cells, Msi-1 may modulate the state of gastric progenitor cells.


Musashi-1 Helicobacter pylori Human Stomach Stem cell 



This study was in part supported by the grants in aid from Japan Society for Promotion of Sciences.


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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Hiroaki Murata
    • 1
    • 2
  • Shingo Tsuji
    • 1
  • Masahiko Tsujii
    • 1
  • Toru Nakamura
    • 2
  • Hai Ying Fu
    • 2
  • Hiroshi Eguchi
    • 2
  • Kayoko Asahi
    • 2
  • Hideyuki Okano
    • 3
  • Sunao Kawano
    • 2
  • Norio Hayashi
    • 1
  1. 1.Department of Gastroenterology and HepatologyOsaka University Graduate School of MedicineSuita, OsakaJapan
  2. 2.Department of Clinical Laboratory Science, School of Allied Health SciencesOsaka University Graduate SchoolOsakaJapan
  3. 3.Department of PhysiologyKeio University School of MedicineTokyoJapan

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