Digestive Diseases and Sciences

, Volume 53, Issue 10, pp 2671–2679 | Cite as

Effect of Lactobacillus casei as a Probiotic on Modulation of Giardiasis

  • Geeta Shukla
  • Pushpa Devi
  • Rakesh Sehgal
Original Paper


The present study was designed with the aim to study the modulation of giardiasis with natural intervention, i.e., Lactobacillus casei––a probiotic, in a murine model. Probiotics are viable microorganisms having a beneficial effect on the prevention and treatment of specific pathological conditions. Various dairy products such as curd, lassi, and kulfi were employed particularly to isolate live organisms (probiotics). Out of five isolated strains only one was found to be similar to a standard strain––Lactobacillus delbrueckii. Experimentally, it was observed that oral feeding of isolated strain A, 7 days prior to inoculation with Giardia lamblia trophozoites, was not as efficient as L. casei in eliminating Giardia infection from BALB/c mice. Interestingly, it was observed that L. casei fed 7 days prior to Giardia infection was more effective and efficient in eliminating the infection from mice. On histological examination, it was observed that probiotic-fed mice had less atrophied villi and infiltrating cells in the small intestine compared to the control. Ultrastructural studies by scanning electron microscopy further confirmed the protection of the mice dosed with L. casei 7 days prior to Giardia infection and also when simultaneously infected with Giardia. It is felt that probiotics, particularly L. casei, modulate Giardia infection by minimizing or preventing the adherence of Giardia trophozoites to the mucosal surface, suggesting that probiotics offer a safe and effective mode to prevent and treat Giardia infection.


Lactobacillus casei Probiotics Giardia lamblia Diarrhea Malabsorption 


  1. 1.
    Craun GF (1996) Waterborne outbreaks of giardiasis : current status. In: SL Erlandsen EA Meyer (eds) Giardia and giardiasis : biology, pathogenesis and epidemiology. New York, Plenum, 243–261Google Scholar
  2. 2.
    Kramer MH, Herwaldt BL, Craun GF, Calderon RL, Juranek DD (1996) Surveillance for waterborne-disease outbreaks—United States, CDC surveillance summaries. Morb Mortal Week Rep 45(SS-1):1–30Google Scholar
  3. 3.
    Walia BN, Ganguly NK, Mahajan RC, Kimar D, Madan IJ, Gambhir SK, Kanwar SS (1986) Morbidity in preschool Giardia cyst excretors. Trop Geograp Med 38(4):367–370Google Scholar
  4. 4.
    Wolfe MS (1992) Giardiasis. Clin Microbiol Rev 5:93–100PubMedGoogle Scholar
  5. 5.
    Khanna R, Vinayak VK, Mehta FS, Kumkum RK, Nain CK (1988) Giardia lamblia infection in immunosuppressed animals causes severe alterations to brush border membrane enzymes. Dig Dis Sci 33:1147–1152PubMedCrossRefGoogle Scholar
  6. 6.
    Eckmann L, Gillin FD (2001) Microbes and microbial toxins: paradigms for microbial-mucosal interactions I. Pathophysiological aspects of enteric infections with the lumen-dwelling protozoan Giardia lamblia. Am J Physiol Gastrointest Liver Physiol 280:G1–G6PubMedGoogle Scholar
  7. 7.
    Calzada F, Cerda GR, Meckes M, Cedillo RR, Bye R, Mata R (1999) Geranins A and B, new antiprotozoal A-typeproanthocyanidins from Geranium niveum. J Nat Prod 62:705–709PubMedCrossRefGoogle Scholar
  8. 8.
    Gardner TB, Hill DR (2001) Treatment of Giardiasis. Clin Microbiol Rev 14:114–128PubMedCrossRefGoogle Scholar
  9. 9.
    Upcroft P, Upcroft JA (2001) Drug targets and mechanisms of resistance in the anaerobic protozoa. Clin Microbiol Rev 14:150–164PubMedCrossRefGoogle Scholar
  10. 10.
    Upcroft JA, Dunn LA, Wright JM, Benakli K, Upcroft P, Vanelle P (2006) 5-Nitroimidazole drug effective against Metronidazole-Resistant Trichomonas vaginalis and Giardia lamblia. Antimicrob Agent Chemother 50(1):344–347PubMedCrossRefGoogle Scholar
  11. 11.
    Kandler O, Weiss N (1986) Regular, Non sporing Gram positive Rods. In Bergey’s manual of systemic bacteriology, Vol 2. Waverly, USA pp 1208–1233 Google Scholar
  12. 12.
    Benyacoub J, Perez PF, Rochat F, Saudan KY, Reuteler G, Antille N, Humen M, De Antoni GL, Cavadani C, Blum S, Schiffrin EJ (2005) Enterococcus faecium SF68 enhances the immune response to Giardia intestinalis in mice. J Nutr 135:1171–1176PubMedGoogle Scholar
  13. 13.
    Vinayak VK, Sharma GL, Naik SR (1979) Experimental Giardia lamblia infectionin Swiss mice––a preliminary report. Indian J Med Res 70:195–198PubMedGoogle Scholar
  14. 14.
    Oyetayo VO, Adetuyi FC, Akinyosoye FA (2003) Safety and protective effect of Lactobacillus acidophilus and Lactobacillus casei used as probiotic agent in vivo. Afr J Biotechnol 2(11):448–452Google Scholar
  15. 15.
    Ney HC (1994) The crisis in antibiotic resistance. Science 257:1064–1073CrossRefGoogle Scholar
  16. 16.
    Tannock GW, Munro HJ, Harmsen GW, Welling SJ, Gopal PK (2002) Analysis of the fecal microflora of human subjects consuming a probiotic product containing Lactobacillus rhamnosus DR 20. Appl Environ Microbiol 66:2578–2588CrossRefGoogle Scholar
  17. 17.
    Lievin-Le MV, Amsellem R, Servin AL, Coconnier MH (2002) Lactobacillus acidophilus (strain LB) from the resident adult human gastrointestinal microflora exerts activity against brush border damage promoted by a diarrhoeagenic Escherichia coli in human enterocyte-like cells. Gut 50:803–811CrossRefGoogle Scholar
  18. 18.
    Bibiloni R, Perez PF, De Antoni GL (1999) Will a high adhering capacity in a probiotic strain guarantee exclusion of pathogens from intestinal epithelia? Anaerobe 5:519–524CrossRefGoogle Scholar
  19. 19.
    Haller D, Blum S, Bode C, Hammes WP, Schiffrin EJ (2000) Activation of human peripheral blood mononuclear cells by nonpathogenic bacteria in vitro: evidence of NK cells as primary targets. Infect Immun 68:752–759PubMedCrossRefGoogle Scholar
  20. 20.
    Benyacoub J, Maulden GL, Cavadini C, Sauthier T, Anderson RE, Schiffrin EJ, Weid T (2003) Supplementation of food with Enterococcus faecium (SF68) stimulates immune functions in young dogs. J Nutr 133:1158–1162PubMedGoogle Scholar
  21. 21.
    Koji N (2005) Prevention of infection by Probiotics. J Biosci Bioeng 100(6):583–592CrossRefGoogle Scholar
  22. 22.
    Humen MA, De Antoni GL, Benyacoub J, Costas MB, Cardozo MI, Kozubsky L, Saudan K, Doenzli-Bruand A, Blum S, Schiffrin EJ, Perez PF (2005) Lactobacillus johnsonii La1 antagonizes Giardia intestinalis in vivo. Infect Immun 73:1265–1269PubMedCrossRefGoogle Scholar
  23. 23.
    Roberts-Thomson IC, Stevens DP, Mahmoud AA, Warren KS (1976) Giardiasis in the mouse: an animal model. Gastroenterol 71:57–61Google Scholar
  24. 24.
    Singer SM, Nash TE (2000) The role of normal flora in Giardia lamblia infections in mice. J Infect Dis 181:1510–1512PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  1. 1.Department of Microbiology, Basic Medical BlockPanjab UniversityChandigarhIndia
  2. 2.Department of ParasitologyPost Graduate Institute of Medical Education and ResearchChandigarhIndia

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