Digestive Diseases and Sciences

, Volume 53, Issue 10, pp 2667–2670 | Cite as

Bioflora Probiotic in Immunomodulation and Prophylaxis of Intestinal Bacterial Translocation in Rats

  • O. M. Laudanno
  • J. A. Cesolari
  • A. Godoy
  • E. Sutich
  • S. Sarangone
  • J. Catalano
  • P. San Miguel
Original Paper


The immunomodulator effect of Bioflora probiotic on T (CD4+) and B (CD20) lymphocytes in gastrointestinal mucosa and intestinal bacterial translocation was studied using Wistar rats (n = 10 per group). Two experiments were used: (I) stress with immobilization and water immersion at 22°C for 7 h plus the application of indomethacin (Indo) 10 mg/kg SC every 24 h for 3 days (comparator group), and (II) stress experiment I with the addition of 1 mL of Bioflora applied through a orogastric tube every 12 h for 3 days. At the 4th day, in asepsis, a dissection laparotomy of liver, spleen, mesenteric lymphatic nodes, and cecum was performed for microbiological culture, and stomach, ileum, and colon were also dissected for immunohistochemical and quantification of CD4+ and CD20. Findings in experiment I revealed cecum bacterial overdevelopment of 6 × 1010 ± 2.3 × 109 colony-forming units (CFU) (P < 0.01) and positive cultures in liver, spleen, and all mesenteric lymphatic nodes. On the other hand, in the group treated with Probiotic Bioflora, cecum without overdevelopment (3 × 106 ± 1.3 × 105 CFU), negative cultures in liver and spleen, and in lymphatic nodes two positive and eight negative cultures for E. coli and P. vulgaris (P < 0.01) were observed. Immunohistochemistry revealed a relevant increase of T lymphocytes (CD4+) in ileum and colon. Conclusions Bioflora probiotic was shown to be an intestinal immunomodulator that induced increased T (CD4+) lymphocytes that also offer prophylaxis of intestinal bacterial translocation in a stressed rat model.


Probiotics Bioflora Bacterial translocation Immunomodulation 



The authors would like to thank Dr. Marta Sofía Cassini for her cooperation in the preparation of this paper.


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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • O. M. Laudanno
    • 1
  • J. A. Cesolari
    • 1
  • A. Godoy
    • 1
  • E. Sutich
    • 1
  • S. Sarangone
    • 1
  • J. Catalano
    • 1
  • P. San Miguel
    • 1
  1. 1.Experimental Gastroenterology, Medical Sciences FacultySchool of Medical SciencesRosarioArgentina

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