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Bioflora Probiotic in Immunomodulation and Prophylaxis of Intestinal Bacterial Translocation in Rats

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Abstract

The immunomodulator effect of Bioflora probiotic on T (CD4+) and B (CD20) lymphocytes in gastrointestinal mucosa and intestinal bacterial translocation was studied using Wistar rats (n = 10 per group). Two experiments were used: (I) stress with immobilization and water immersion at 22°C for 7 h plus the application of indomethacin (Indo) 10 mg/kg SC every 24 h for 3 days (comparator group), and (II) stress experiment I with the addition of 1 mL of Bioflora applied through a orogastric tube every 12 h for 3 days. At the 4th day, in asepsis, a dissection laparotomy of liver, spleen, mesenteric lymphatic nodes, and cecum was performed for microbiological culture, and stomach, ileum, and colon were also dissected for immunohistochemical and quantification of CD4+ and CD20. Findings in experiment I revealed cecum bacterial overdevelopment of 6 × 1010 ± 2.3 × 109 colony-forming units (CFU) (P < 0.01) and positive cultures in liver, spleen, and all mesenteric lymphatic nodes. On the other hand, in the group treated with Probiotic Bioflora, cecum without overdevelopment (3 × 106 ± 1.3 × 105 CFU), negative cultures in liver and spleen, and in lymphatic nodes two positive and eight negative cultures for E. coli and P. vulgaris (P < 0.01) were observed. Immunohistochemistry revealed a relevant increase of T lymphocytes (CD4+) in ileum and colon. Conclusions Bioflora probiotic was shown to be an intestinal immunomodulator that induced increased T (CD4+) lymphocytes that also offer prophylaxis of intestinal bacterial translocation in a stressed rat model.

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Acknowledgement

The authors would like to thank Dr. Marta Sofía Cassini for her cooperation in the preparation of this paper.

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Authors and Affiliations

  1. Experimental Gastroenterology, Medical Sciences Faculty, School of Medical Sciences, Cochabamba 2090, Rosario, Argentina

    O. M. Laudanno, J. A. Cesolari, A. Godoy, E. Sutich, S. Sarangone, J. Catalano & P. San Miguel

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  1. O. M. Laudanno
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  2. J. A. Cesolari
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  3. A. Godoy
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  4. E. Sutich
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  5. S. Sarangone
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  6. J. Catalano
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  7. P. San Miguel
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Correspondence to O. M. Laudanno.

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Laudanno, O.M., Cesolari, J.A., Godoy, A. et al. Bioflora Probiotic in Immunomodulation and Prophylaxis of Intestinal Bacterial Translocation in Rats. Dig Dis Sci 53, 2667–2670 (2008). https://doi.org/10.1007/s10620-007-0179-5

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  • DOI: https://doi.org/10.1007/s10620-007-0179-5

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