Advertisement

Digestive Diseases and Sciences

, Volume 53, Issue 6, pp 1582–1588 | Cite as

Usefulness of Antibodies to Deamidated Gliadin Peptides in Celiac Disease Diagnosis and Follow-up

  • Umberto Volta
  • Alessandro Granito
  • Erica Fiorini
  • Claudia Parisi
  • Maria Piscaglia
  • Georgios Pappas
  • Paolo Muratori
  • Francesco B. Bianchi
Original Paper

Abstract

The prevalence of the recently described deamidated gliadin peptide antibodies was compared with that of the routinely used antigliadin, antiendomysial, and tissue transglutaminase antibodies in the sera of 128 untreated celiac patients and 134 controls. Sensitivity and specificity for celiac disease were 83.6 and 90.3% for IgA and 84.4 and 98.5% for IgG antibodies to deamidated gliadin peptides. The new test displayed higher diagnostic accuracy than antigliadin antibodies and, although less sensitive than antiendomysial and tissue transglutaminase antibodies, showed significantly higher specificity than tissue transglutaminase antibodies (P < 0.001). Persistence of peptide antibodies after gluten withdrawal was an expression of low compliance with the diet and of the lack of improvement of the intestinal mucosa. The combined use of tissue transglutaminase and deamidated gliadin peptide antibodies seems to be a very useful tool for celiac disease diagnosis. Moreover, antibodies to deamidated gliadin peptides can be helpful in disease follow-up.

Keywords

Antibodies to deamidated gliadin peptides Celiac disease Diagnosis Follow-up 

References

  1. 1.
    Alaedini A, Green PHR (2005) Narrative review Coeliac disease: understanding a complex autoimmune disorder. Ann Int Med 142:289–298PubMedGoogle Scholar
  2. 2.
    Volta U, Rodrigo L, Granito A, Petrolini N, Muratori P, Muratori L, Linares A, Veronesi L, Fuentes D, Zauli D, Bianchi FB (2002) Celiac disease in autoimmune cholestatic liver disorders. Am J Gastroenterol 97:2609–2613PubMedCrossRefGoogle Scholar
  3. 3.
    Catassi C, Kryszak D, Louis-Jacques O, Duerksen DR, Hill I, Crowe SE, Brown AR, Procaccini NJ, Wonderly BA, Hartley P, Moreci J, Bennett N, Horvath K, Burk M, Fasano A (2007) Detection of celiac disease in primary care: a multicenter case-finding study in North America. Am J Gastroenterol 102:1–7CrossRefGoogle Scholar
  4. 4.
    Bizzaro N, Tampoia M, Villalta D, Platzgummer S, Liguori M, Tozzoli R, Tonutti E (2006) Low specificity of anti-tissue transglutaminase antibodies in patients with primary biliary cirrhosis. J Clin Lab Anal 20:184–189PubMedCrossRefGoogle Scholar
  5. 5.
    Tonutti E, Visentini D, Bizzaro N, Caradonna M, Cerni L, Villalta D, Tozzoli R (2003) The role of antitissue transglutaminase assay for the diagnosis and monitoring of coeliac disease: a French-Italian multicentre study. J Clin Pathol 56:389–393PubMedCrossRefGoogle Scholar
  6. 6.
    Murray JA, Herlein J, Mitros F, Goeken JA (2000) Serologic testing for celiac disease in the United States: results of a multilaboratory comparison study. Clin Diagn Lab Immunol.7:584–587PubMedCrossRefGoogle Scholar
  7. 7.
    Hill ID (2005) What are the sensitivity and specificity of serologic tests for celiac disease? Do sensitivity and specificity vary in different populations? Gastroenterology 128(4 suppl 1):S25–S32PubMedCrossRefGoogle Scholar
  8. 8.
    Burgin-Wolff A, Gaze H, Hadziselimovic F, Huber H, Lentze MJ, Nussle D, Reymond-Berthet C (1991) Antigliadin and antiendomysium antibody determination for coeliac disease. Arch Dis Child.66:941–947PubMedCrossRefGoogle Scholar
  9. 9.
    Korponay-Szabò IR, Dahlbom I, Laurila J, Koskinen S, Woolley N, Partanen J, Kovacs JB, Maki M, Hansson T (2003) Elevation of IgG antibodies against tissue transglutaminase as a diagnostic tool for coeliac disease in selective IgA deficiency. Gut 52:1567–1571PubMedCrossRefGoogle Scholar
  10. 10.
    Ciccocioppo R, Di Sabatino A, Ara C, Biagi F, Perilli M, Amicosante G, Cifone MG, Corazza GR (2003) Gliadin and tissue transglutaminase complexes in normal and coeliac duodenal mucosa. Clin Exp Immunol 134:516–524PubMedCrossRefGoogle Scholar
  11. 11.
    Molberg O, McAdam S, Korner R, Quarsten H, Kristiansen C, Madsen L, Fugger L, Scott H, Noren O, Roepstorff P, Lundin KE, Sjostrom H, Sollid LM (1998) Tissue transglutaminase selectively modifies gliadin peptides that are recognized by gut-derived T cells in celiac disease. Nat Med 4:713–717PubMedCrossRefGoogle Scholar
  12. 12.
    van de Wal Y, Kooy Y, van Veelen P, Pena S, Mearin L, Papadopoulos G, Koning F (1998) Selective deamidation by tissue transglutaminase strongly enhances gliadin-specific T cell reactivity. J Immunol 161:1585–1588PubMedGoogle Scholar
  13. 13.
    Osman AA, Gunnel T, Dietl A, Uhlig HH, Amin M, Fleckenstein B, Richter T, Mothes T (2000) B cell epitopes of gliadin. Clin Exp Immunol 121:248–254PubMedCrossRefGoogle Scholar
  14. 14.
    Aleanzi M, Demonte AM, Esper C, Garcilazo S, Waggener M (2001) Celiac disease: antibody recognition against native and selectively deamidated gliadin peptides. Clin Chem.47:2023–2028PubMedGoogle Scholar
  15. 15.
    Prince HE (2006) Evaluation of the INOVA diagnostics enzyme-linked immunosorbent assay kits for measuring serum immunoglobulin G (IgG) and IgA to deamidated gliadin peptides. Clin Vacc Immunol 13:150–151CrossRefGoogle Scholar
  16. 16.
    Sugai E, Vazquez H, Nachman F, Moreno ML, Mazure R, Smecuol E, Niveloni S, Cabanne A, Kogan Z, Gomez JC, Maurino E, Bai JC (2006) Accuracy of testing for antibodies to synthetic gliadin-related peptides in celiac disease. Clin Gastroenterol Heptol 4:1112–1117CrossRefGoogle Scholar
  17. 17.
    Oberhuber G, Granditsch G, Vogelsang H (1999) The histopathology of coeliac disease: time for a standardized report scheme for pathologists. Eur J Gastroenterol Hepatol 11:1185–1194PubMedCrossRefGoogle Scholar
  18. 18.
    Berger R, Schmidt G (1996) Evaluation of six anti-gliadin antibody assays. J Immunol Methods 191:77–86PubMedCrossRefGoogle Scholar
  19. 19.
    Volta U, Molinaro N, De Franceschi L, Fratangelo D, Bianchi FB (1995) IgA anti-endomysial antibodies on human umbilical cord tissue for celiac disease: save both money and monkeys. Dig Dis Sci 40:1902–1905PubMedCrossRefGoogle Scholar
  20. 20.
    Fasano A, Catassi C (2001) Current approaches to diagnosis and treatment of celiac disease: an evolving spectrum. Gastroenterology 120:636–651PubMedCrossRefGoogle Scholar
  21. 21.
    Green PHR, Jabri B (2003) Coeliac disease. Lancet 362:383–391PubMedCrossRefGoogle Scholar
  22. 22.
    Stern M (2000) Comparative evaluation of serologic tests for celiac disease: a European initiative towards standardization. J Ped Gastroenterol Nutr 31:513–519CrossRefGoogle Scholar
  23. 23.
    Bizzaro N, Villalta D, Tonutti E, Doria A, Tampoia M, Bassetti D, Tozzoli R (2003) IgA and IgG tissue transglutaminase antibody prevalence and clinical significance in connective tissue diseases, inflammatory bowel disease and primary biliary cirrhosis. Dig Dis Sci 48:2360–2365PubMedCrossRefGoogle Scholar
  24. 24.
    Granito A, Muratori L, Muratori P, Petrolini N, Bianchi FB, Volta U (2004) Antitransglutaminase antibodies and giardiasis. Am J Gastrorenterol 99:2505–2506CrossRefGoogle Scholar
  25. 25.
    Sardy M, Csikos M, Geisen C, Preisz K, Kornsee Z, Tomsits E, Tox U, Hunzelmann N, Wieslander J, Karpati S, Paulsson M, Smyth N (2007) Tissue transglutaminase ELISA positivity in autoimmune disease independent of gluten-sensitive disease. Clin Chim Acta 376:126–135PubMedCrossRefGoogle Scholar
  26. 26.
    Sugai E, Smecuol E, Niveloni S, Vazquez H, Label M, Mazure R, Czech A, Kogan Z, Maurino E, Bai JC (2006) Celiac disease serology in dermatitis herpetiformis: which is the best option for detecting gluten sensitivity? Acta Gastroenterol Latinoam 36:197–201PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Umberto Volta
    • 1
  • Alessandro Granito
    • 1
  • Erica Fiorini
    • 1
  • Claudia Parisi
    • 1
  • Maria Piscaglia
    • 1
  • Georgios Pappas
    • 1
  • Paolo Muratori
    • 1
  • Francesco B. Bianchi
    • 1
  1. 1.Department of Gastroenterology and Internal Medicine, S. Orsola-Malpighi HospitalUniversity of BolognaBolognaItaly

Personalised recommendations