Molecular Analysis of the p53 Gene in Patients with Intestinal Metaplasia of the Cardia and Barrett's Esophagus: Characterization by Sequencing
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To the Editor: Contrary to Dr. Novotna's findings (Digestive Disease and Sciences, Vol. 51, No. 1, pp. 110–113, January 2006), we believe that TP53 gene mutations can occur before transition from low-grade to high-grade dysplasia. In this sense, we analyze the main molecular alterations present in exons 5 to 8 of TP53 in patients with intestinal metaplasia of the cardia (IMC) and Barrett's esophagus (BE) without displasia. Sixteen patients were evaluated (eight women and eight men; ages, 23 to 72 years; average, 54.3 years). These individuals had GERD symptoms diagnosed by upper digestive endoscopy and confirmed by biopsy. The biopsies were analyzed as described by Segal et al. . Patients had DNA extracted from 10 ml of peripheral blood collected in EDTA using the modified salt precipitation technique described by Miller et al. . The same protocol was performed with respect to tissue samples collected at the time of endoscopy. The long PCR technique of exons 5 to 8 of the p53...
KeywordsMissense Mutation Esophagitis Genetic Predisposition Intestinal Metaplasia Molecular Alteration
This work received editorial support from the Research and Postgraduate Group of Hospital de Clínicas in Porto Alegre (RS, Brazil) and financial support from the Research Support Foundation of Rio Grande do Sul (FAPERGS), Coordination for the Improvement of Higher Education Personnel (CAPES), and Research Support Fund/Hospital de Clínicas in Porto Alegre (FIPE-HCPA).
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