Abstract
Our aim was to study the effect of a mucosal protective agent, rebamipide, on the colonic barrier and the immune response in colitis-prone interleukin-10-deficient (IL-10−/−) C57BL/6 mice infected with Helicobacter hepaticus. After sacrifice, in all mice, control, or previously infected with H. hepaticus, or previously infected and treated with rebamipide enema, a histological examination of colonic samples was performed, intestinal permeability was studied in Ussing chamber, and mesenteric lymph node proliferation and cytokine secretion were measured. Mice treated with rebamipide presented a reinforcement of the distal colonic epithelial barrier, an increase of mesenteric lymph node cells proliferation, and of IFNγ and IL-12 secretion. These results indicate that in IL-10−/− mice with mild colitis, rectally administered rebamipide reinforces the distal colonic barrier and has a slight Th1 immuno-stimulatory effect on mesenteric lymph node cells. These properties could be helpful in the management of some inflammatory bowel diseases.
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This work was supported by Otsuka Pharmaceutical, Tokyo, Japan.
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Laharie, D., Ménard, S., Asencio, C. et al. Effect of Rebamipide on the Colonic Barrier in Interleukin-10-Deficient Mice. Dig Dis Sci 52, 84–92 (2007). https://doi.org/10.1007/s10620-006-9183-4
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DOI: https://doi.org/10.1007/s10620-006-9183-4