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Digestive Diseases and Sciences

, Volume 52, Issue 1, pp 84–92 | Cite as

Effect of Rebamipide on the Colonic Barrier in Interleukin-10-Deficient Mice

  • David Laharie
  • Sandrine Ménard
  • Corinne Asencio
  • Teresita Vidal-Martinez
  • Anne Rullier
  • Frank Zerbib
  • Céline Candalh
  • Francis Mégraud
  • Martine Heyman
  • Tamara Matysiak-Budnik
Original Paper

Abstract

Our aim was to study the effect of a mucosal protective agent, rebamipide, on the colonic barrier and the immune response in colitis-prone interleukin-10-deficient (IL-10−/−) C57BL/6 mice infected with Helicobacter hepaticus. After sacrifice, in all mice, control, or previously infected with H. hepaticus, or previously infected and treated with rebamipide enema, a histological examination of colonic samples was performed, intestinal permeability was studied in Ussing chamber, and mesenteric lymph node proliferation and cytokine secretion were measured. Mice treated with rebamipide presented a reinforcement of the distal colonic epithelial barrier, an increase of mesenteric lymph node cells proliferation, and of IFNγ and IL-12 secretion. These results indicate that in IL-10−/− mice with mild colitis, rectally administered rebamipide reinforces the distal colonic barrier and has a slight Th1 immuno-stimulatory effect on mesenteric lymph node cells. These properties could be helpful in the management of some inflammatory bowel diseases.

Keywords

Rebamipide Intestinal permeability Colitis Interleukin 10 Immune response 

Notes

Acknowledgment

This work was supported by Otsuka Pharmaceutical, Tokyo, Japan.

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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • David Laharie
    • 1
  • Sandrine Ménard
    • 2
  • Corinne Asencio
    • 1
  • Teresita Vidal-Martinez
    • 2
  • Anne Rullier
    • 3
  • Frank Zerbib
    • 1
  • Céline Candalh
    • 2
  • Francis Mégraud
    • 1
  • Martine Heyman
    • 2
  • Tamara Matysiak-Budnik
    • 2
    • 4
  1. 1.INSERM-ERI 10Université Victor SegalenBordeauxFrance
  2. 2.INSERM U793Faculté Necker-René DescartesBordeauxFrance
  3. 3.Service d’Anatomo-pathologieHôpital PellegrinBordeauxFrance
  4. 4.INSERM U793Faculté Necker-René DescartesParisFrance

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