Non-Helicobacter Bacterial Flora Rarely Develops in the Gastric Mucosal Layer of Children
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Non-Helicobacter bacteria can be cultured from the gastric mucosa in adults but in children, there are no studies about such microflora. The purpose of this study, therefore, was to clarify whether gastric biota develops in children. In 10 children and 10 adults or elderly (5 H. pylori-infected and 5 uninfected in each group), biopsy specimens of the gastric antrum and corpus and gastric juice were studied for bacterial examinations and the data were compared between both age groups in relation to H. pylori status and luminal pH. Bacterial genera and species were analyzed using both culture and real-time polymerase chain reaction (PCR) with the 52 genus- and species-specific primer sets. Non-Helicobacterbacteria in the mucosa were cultured from all adult patients, whereas microorganisms were cultured in only one child (p < .001). Gastric pH was lower in children (median, 1.4) than in adults (median, 2.6) (p < .005). The grade of endoscopic gastric atrophy was moderate or severe in 8 adults, but absent or mild in all 10 children. Among adults, there was a significant positive correlation between gastric pH and total bacterial counts of both the mucosa and juice. These data indicate that impaired gastric acid secretion associated with long-term H. pylori infection enables non-Helicobacter bacteria to colonize in the human stomach. Such microorganisms rarely colonize in the gastric mucosa in children regardless of H. pylori status.
Key Wordschild gastric acid gastric flora Helicobacter pylori PCR
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- 1.Guarner F, Malagelada JR: Gut flora in health and disease. Lancet 360:512–519, 2003Google Scholar
- 4.Sandulean S, Jonkers D, De Bruine A, Hameeteman W, Stockbrugger RW: Non-Helicobacter pylori bacterial flora during acid-suppressive therapy: differential findings in gastric juice and gastric mucosa. Aliment Pharmacol Ther 15:379–388, 2001Google Scholar
- 7.Adamsson I, Nord CE, Lundquist P, Sjöstedt S, Edlund C: Comparative effects of omeprazole, amoxicillin plus metronidazole versus omeprazole, clarithromycin plus metronidazole on the oral, gastric and intestinal microflora in Helicobacter pylori-infected patients. J Antimicrob Chemother 44:629–640, 1999CrossRefPubMedGoogle Scholar
- 8.Houben GM, Stockbrügger RW: Bacteria in the aetio-pathogenesis of gastric cancer: a review. Scand J Gastroenterol 212(Suppl):13–18, 1995Google Scholar
- 11.Monstein HJ, Tiveljung A, Kraft CH, Borch K, Jonasson J: Profiling of bacterial flora in gastric biopsies from patients with Helicobacter pylori-associated gastritis and histologically normal control individuals by temperature gradient gel electrophoresis and 16S rDNA sequence analysis. J Med Microbiol 49:817–822, 2000PubMedGoogle Scholar
- 14.Sharma BK, Santana IA, Wood EC, et al.: Intragastric bacterial activity and nitrosation before, during, and after treatment with omeprazole. Br Med J 289:717–719, 1984Google Scholar
- 27.Song Y., Kato N, Liu CX, Matsumiya Y, Kato H, Watanabe K: Rapid identification of 11 human intestinal Lactobacillus species by multiplex PCR assays using group- and species- specific primers derived from the 16S-23S rRNA intergenic spacer region and its flanking 23S rRNA. FEMS Microbiol Lett 187:167–173, 2000PubMedGoogle Scholar
- 32.Wilder-Smith CH, Spirig C, Krech T, Merki HS: Bactericidal factors in gastric juice. Eur J Gastroenterol Hepatol 4:885–891, 1992Google Scholar