Digestive Diseases and Sciences

, Volume 51, Issue 1, pp 178–184 | Cite as

Flow Cytometric Analysis of Expression of Transforming Growth Factor-β and Glucocorticoid-Induced Tumor Necrosis Factor Receptor on CD4+ CD25+ T Cells of Patients with Inflammatory Bowel Disease

  • Maho Ikeda
  • Fuminao Takeshima
  • Kazuo Ohba
  • Ken Ohnita
  • Hajime Isomoto
  • Masaki Yamakawa
  • Katsuhisa Omagari
  • Yohei Mizuta
  • Shigeru Kohno


To determine whether human CD4+CD25+ cells express glucocorticoid-induced tumor necrosis factor receptor (GITR) and transforming growth factor-β (TGF-β) and the difference in CD4+CD25+ cells between patients with inflammatory bowel diseases and healthy subjects, peripheral blood lymphocytes were obtained from patients with ulcerative colitis (UC; n = 50), Crohn's disease (CD; n = 49), and healthy volunteers (control; n = 50) and flow cytometric analysis was performed. In control subjects, the expression of GITR on CD4+CD25+ cells (41.8 ± 10.5%) was significantly higher than on CD4+CD25– cells (11.1 ± 7.4%). Similarly, TGF-β expression on CD4+CD25+ cells (5.3 ± 4.6%) was higher than on CD4+CD25– cells (1.2 ± 1.4%). There were no significant differences among UC, CD, and control in CD4+CD25+/CD4+ ratio. However, there was a significant difference in the CD4+CD25+TGF-β+/CD4+CD25+ ratio between active UC and inactive UC (2.7 ± 2.6 and 7.2 ± 3.9%, respectively). The results suggest that TGF-β is involved in the induction or sustained remission of UC.

Key Words

inflammatory bowel disease regulatory T cell transforming growth factor-β 


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Copyright information

© Springer Science + Business Media, Inc. 2006

Authors and Affiliations

  • Maho Ikeda
    • 1
  • Fuminao Takeshima
    • 2
    • 4
  • Kazuo Ohba
    • 1
  • Ken Ohnita
    • 1
  • Hajime Isomoto
    • 1
  • Masaki Yamakawa
    • 3
  • Katsuhisa Omagari
    • 1
  • Yohei Mizuta
    • 1
  • Shigeru Kohno
    • 1
  1. 1.Second Department of Internal MedicineNagasaki University School of MedicineNagasakiJapan
  2. 2.Department of General MedicineNagasaki University School of MedicineNagasakiJapan
  3. 3.Department of Internal MedicineNagasaki Municipal HospitalNagasakiJapan
  4. 4.Department of General MedicineNagasaki University School of MedicineNagasakiJapan

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