Flow Cytometric Analysis of Expression of Transforming Growth Factor-β and Glucocorticoid-Induced Tumor Necrosis Factor Receptor on CD4+ CD25+ T Cells of Patients with Inflammatory Bowel Disease
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To determine whether human CD4+CD25+ cells express glucocorticoid-induced tumor necrosis factor receptor (GITR) and transforming growth factor-β (TGF-β) and the difference in CD4+CD25+ cells between patients with inflammatory bowel diseases and healthy subjects, peripheral blood lymphocytes were obtained from patients with ulcerative colitis (UC; n = 50), Crohn's disease (CD; n = 49), and healthy volunteers (control; n = 50) and flow cytometric analysis was performed. In control subjects, the expression of GITR on CD4+CD25+ cells (41.8 ± 10.5%) was significantly higher than on CD4+CD25– cells (11.1 ± 7.4%). Similarly, TGF-β expression on CD4+CD25+ cells (5.3 ± 4.6%) was higher than on CD4+CD25– cells (1.2 ± 1.4%). There were no significant differences among UC, CD, and control in CD4+CD25+/CD4+ ratio. However, there was a significant difference in the CD4+CD25+TGF-β+/CD4+CD25+ ratio between active UC and inactive UC (2.7 ± 2.6 and 7.2 ± 3.9%, respectively). The results suggest that TGF-β is involved in the induction or sustained remission of UC.
Key Wordsinflammatory bowel disease regulatory T cell transforming growth factor-β
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