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Digestive Diseases and Sciences

, Volume 50, Issue 8, pp 1497–1505 | Cite as

The Effect of Digestion of Collagen and Elastin on Histomorphometry and the Zero-Stress State in Rat Esophagus

  • Yanhua Fan
  • Jingbo Zhao
  • Donghua Liao
  • Hans Gregersen
Article

Abstract

To understand the physiology and pathology of the esophagus, it is necessary to know the mechanical properties and their dependence on the structural components. The aim of this study was to investigate the effect of collagenase and elastase on the morphological and biomechanical properties in the no-load and zero-stress states in the rat esophagus. Twenty tissue rings from each esophagus of seven normal rats were sectioned in an organ bath containing calcium-free Krebs solution with dextran and EGTA. After the rings were photographed in the no-load state, 8 of the 20 rings were separated into mucosa–submucosa and muscle rings and the rings were transferred to four different solutions containing collagenase, elastase, or corresponding control solutions. The rings were cut radially to obtain the zero-stress state and photographed again. The thickness, area, and opening angle were measured from the digitized images. The collagen and elastin area fractions were determined from histological slides with Van Gieson and Weigert’s elastic stain. The opening angles and residual strain did not differ in the nonseparated enzyme-treated rings and control rings. However, in the separated mucosa–submucosa ring the opening angle was significantly smaller after treatment than that in control rings (P < 0.01). Collagenase and elastase reduced collagen and elastin in the mucosa–submucosa layer about 40% in the nonseparated wall and 54% in the separated mucosa–submucosa layer (P < 0.01). Collagenase and elastase increased the thickness in the separated mucosa–submucosa layer compared to the control (P < 0.05). Disconnection between the epithelia and the lamina propria was histologically observed after elastase digestion. In conclusion, collagenase and elastase caused the opening angle and the residual strain in the separated mucosa–submucosa layer to decrease. The opening angle of the separated mucosa–submucosa layer depended to some extent on the fraction of collagen and elastin.

Key Words

rat esophagus opening angle zero stress residual strain collagenase elastase 

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References

  1. 1.
    Christensen J: The oesophagus. In Physiology of the Gastrointestinal Tract. Johnson LR, Christensen J, Jackson MJ, Jacobson ED, JH Walsh (eds, 2nd edn), New York, Raven Press, 1987Google Scholar
  2. 2.
    Christensen J, Freeman BW, Miller JK: Some physiological characteristics of the esophagogastric junction in the opossum. Gastroenterology 64:1119–1125, 1973PubMedGoogle Scholar
  3. 3.
    Orberg JW, Klein L, Hiltner A: Scanning electron microscopy of collagen fibers in intestine. Connect Tissue Res 9:187–193, 1982PubMedGoogle Scholar
  4. 4.
    Gabella G: Structure of muscles and nerves in the GI tract. In Physiology of the Gastrointestinal Tract. Johnson LR, Christensen J, Jackson MJ, Jacobson ED, JH Walsh (eds, 2nd edn), New York, Raven Press,1987Google Scholar
  5. 5.
    Schulze K, Ellerbroek S, Martin J: Matrix composition in opossum esophagus. Dig Dis Sci 46:968–975, 2001CrossRefPubMedGoogle Scholar
  6. 6.
    Gregersen H, Giversen IM, Rasmussen LM, Tøttrup A: Biomechanical wall properties and collagen content in the partially obstructed opossum esophagus. Gastroenterology 103:1547–1551, 1992PubMedGoogle Scholar
  7. 7.
    Vinter-Jensen L, Juhl CO, Gregersen H: Regional differences in passive elastic wall properties of the esophagus: An impedance planimetric study in pigs. Neurogastroenterol Motil 6:233–238, 1994Google Scholar
  8. 8.
    Gregersen H, Kassab GS: Biomechanics of the gastrointestinal tract. Neurogastroenterol Motil 8:277–297, 1996PubMedGoogle Scholar
  9. 9.
    Gregersen H, Lee TC, Chien S, Skalak R, Fung YC: Strain Distribution in the Layered Wall of the Esophagus. J Biomech Eng 121:442–448, 1999PubMedGoogle Scholar
  10. 10.
    Gregersen H, Kassab GS, Fung YC: The zero-stress state of the gastrointestinal tract: biomechanical and functional implications. Dig Dis Sci 45:2271–2281, 2000CrossRefPubMedGoogle Scholar
  11. 11.
    Gregersen H, Weis SM, Mcculloch AD: Oesophageal morphometry and residual strain in a mouse model of osteogenesis imperfecta. Neurogastroenterol Motil 13:457–464, 2001CrossRefPubMedGoogle Scholar
  12. 12.
    Gregersen H: Biomechanics of the Gastrointestinal Tract. London, Springer-Verlag, 2002Google Scholar
  13. 13.
    Zhao J, Yang J, Zhuang FY, Gregersen H: Biomechanical properties of esophagus during systemic treatment with epidermal growth factor in rats. Ann Biomed Eng 31:700–709, 2003CrossRefPubMedGoogle Scholar
  14. 14.
    Lu X, Gregersen H: Regional distribution of axial strain and circumferential residual strain in the layered rabbit esophagus. J Biomech 34:225–233, 2001PubMedGoogle Scholar
  15. 15.
    Liao D, Fan Y, Zeng Y, Gregersen H: Stress distribution in the layered wall of the rat oesophagus. Med Eng Phys 25:731–738, 2003CrossRefPubMedGoogle Scholar
  16. 16.
    Hoffman AS, Grande LA, Park JB: Sequential enzymolysis of human aorta and resultant stress-strain behavior. Biomater Med Devices Artif Organs 5:121–145, 1977PubMedGoogle Scholar
  17. 17.
    Kitoh T, Kawai Y, Ohhashi T: Effects of collagenase, elastase, and hyaluronidase on mechanical properties of isolated dog jugular veins. Am J Physiol 265(1 Pt 2):H273–H280, 1993PubMedGoogle Scholar
  18. 18.
    Zeller PJ, Skalak TC: Contribution of individual structural components in determining the zero-stress state in small arteries. J Vasc Res 35:8–17, 1998PubMedGoogle Scholar
  19. 19.
    Dobrin PB, Baker WH, Gley WC: Elastolytic and collagenolytic studies of arteries. Implications for the mechanical properties of aneurysms. Arch Surg 119:405–409, 1984PubMedGoogle Scholar
  20. 20.
    Omens JH, Rockman HA, Covell JW: Passive ventricular mechanics in tight-skin mice. Am J Physiol 266:H1169–H1176, 1994PubMedGoogle Scholar
  21. 21.
    Rodriguez-Revenga L, Iranzo P, Badenas C, Puig S, Carrio A, Mila M: A novel elastin gene mutation resulting in an autosomal dominant form of cutis laxa. Arch Dermatol 140:1135–1139, 2004PubMedGoogle Scholar
  22. 22.
    Mimura T, Emanuel A, Kamm MA: Pathophysiology of diverticular disease. Best Pract Res Clin Gastroenterol 16:563–576, 2002PubMedGoogle Scholar
  23. 23.
    Zanetti M, Braghetta P, Sabatelli P, Mura I, Doliana R, Colombatti A, Volpin D, Bonaldo P, Bressan GM: EMILIN-1 deficiency induces elastogenesis and vascular cell defects. Mol Cell Biol 24:638–650, 2004PubMedGoogle Scholar

Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  • Yanhua Fan
    • 1
    • 2
  • Jingbo Zhao
    • 1
    • 3
  • Donghua Liao
    • 1
    • 3
  • Hans Gregersen
    • 1
    • 3
    • 4
    • 5
  1. 1.Center of Excellence in Visceral Biomechanics and PainAalborg HospitalAalborgDenmark
  2. 2.Department of GastroenterologyChina-Japan Friendship HospitalBeijingChina
  3. 3.Center of Sensory-Motor InteractionAalborg UniversityAalborgDenmark
  4. 4.La Jolla Bioengineering InstituteLa Jolla
  5. 5.Center of Excellence in Visceral Biomechanics and PainAalborg HospitalAalborgDenmark

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