Malignant Transformation of the Mouse Anorectal Epithelium Induced by an Inoculated Human Cancer Cell Line
Four kinds of human cancer cell lines and one mouse caner cell line were inoculated into the subepithelial area of the anorectum of female nude mice. Among the cell lines, two cell lines (KATO III and Lu 135) showed the potential enforcement of atypical changes in the adjacent mouse anorectal epithelium. Moreover, the submucosal invasion of the malignant transformed cells of the mouse epithelium was demonstrated in specimens obtained from three KATO III-inoculated mice. This exciting and novel phenomenon clearly demonstrates the need to change the present general concept of a single-cell origin of cancer tissue. This valuable and novel discovery may change the basis of oncology research while also providing new ideas for projects to investigate the mechanisms of carcinogenesis from several aspects such as molecular biology, cell biology, and pathology. Moreover, the novel experimental design itself is also extremely useful as a simple model for investigating the mechanisms of oncogenesis.
Unable to display preview. Download preview PDF.
- 1.Alberts B, Bray D, Lewis J, Raff M, Roberts K, Watson JD: Molecular Biology of the Cell, 3rd ed. New York, London, Garland, 1994, pp 1275–1294Google Scholar
- 3.Nowell PC, Hungerford DA: A minute chromosome in human chronic granulocytic leukemia. Science 132:1497, 1960Google Scholar
- 5.Fialkow PJ: Clonal origin of human tumors. Biochim Biophys Acta 12;458(3):283–321, 1976Google Scholar
- 6.Tatematsu M, Masui T, Fukami H, et al.: Primary monoclonal and secondary polyclonal growth of colon neoplastic lesions in C3H/HeN → BALB/c chimeric mice treated with 1,2-dimethylhydrazine immunohistochemical detection of C3H strain-specific antigen and simple sequence length polymorphism analysis of DNA. Int J Cancer 66(2):234–238, 1996PubMedGoogle Scholar