Hematopoietic stem cell and mesenchymal stem cell population size in bone marrow samples depends on patient’s age and harvesting technique
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Mesenchymal stem cells (MSCs) are heterogeneous population of cells with great potential for regenerative medicine. MSCs are relatively easy to expand in a cell culture, however determination of their concentration in harvested tissue is more complex and is not implemented as routine procedure. To identify MSCs collected from bone marrow we have used two combinations of cell markers (CD45−/CD73+/CD90+/CD105+ and CD45−/CD271+) and fibroblast colony-forming unit (CFU-F) assay. Further, in donors of various ages, mesenchymal stem cell concentration was compared with the result of CFU-F assay and with hematopoietic stem cell concentration, determined by a standardized flow cytometric assay. A positive correlation of MSC populations to the CFU-F numbers is observed, the population of the CD45−/CD271+ cells correlates better with CFU-F numbers than the population of the CD45−/CD73+/CD90+/CD105+ cells. The relationship between the hematopoietic CD45dim/CD34+ cell concentration and mesenchymal CFU-Fs or CD45−/CD271+ cells shows a positive linear regression. An age-related quantitative reduction of hematopoietic CD45dim/CD34+, mesenchymal CD45−/CD73+/CD90+/CD105+ and CD45−/CD271+ stem cells, and CFU-F numbers were noted. Additionally, statistically significant higher CFU-F numbers were observed when bone marrow samples were harvested from three different sites from the anterior iliac crest instead of harvesting the same sample amount only from one site.
KeywordsMesenchymal stem cells CD271+ cells Bone marrow CFU-F assay Hematopoietic stem cells
The work was supported by the Slovenian Research Agency (Grant No. P3-0371).
- Alvarez-Viejo M, Menendez-Menendez Y, Blanco-Gelaz MA et al (2013) LNGFR (CD271) as marker to identify mesenchymal stem cells from different human sources: Umbilical cord blood, Wharton’s jelly and bone marrow. J Bone Marrow Res 1:132Google Scholar
- Koç ON, Gerson SL, Cooper BW, Dyhouse SM, Haynesworth SE, Caplan AI, Lazarus HM (2000) Rapid hematopoietic recovery after coinfusion of autologous-blood stem cells and culture-expanded marrow mesenchymal stem cells in advanced breast cancer patients receiving high-dose chemotherapy. J Clin Oncol 18(2):307–316CrossRefGoogle Scholar
- Prasad VK, Lucas KG, Kleiner GI et al (2010) Efficacy and safety of ex vivo cultured adult human mesenchymal stem cells (Prochymal) in pediatric patients with severe refractory acute graft-versus-host disease in a compassionate use study. Biol Blood Marrow Transpl 17(4):534–541CrossRefGoogle Scholar