Abstract
Recent developments in bone tissue engineering have paved the way for more efficient and cost-effective strategies. Additionally, utilization of autologous sources has been considered very desirable and is increasingly growing. Recently, activated platelet rich plasma (PRP) has been widely used in the field of bone tissue engineering, since it harbours a huge number of growth factors that can enhance osteogenesis and bone regeneration. In the present study, the osteogenic effects of PRP coated nanofibrous PES/PVA scaffolds on adipose-derived mesenchymal stem cells have been investigated. Common osteogenic markers were assayed by real time PCR. Alkaline phosphate activity, calcium deposition and Alizarin red staining assays were performed as well. The results revealed that the highest osteogenic differentiation occurred when cells were cultured on PRP coated PES/PVA scaffolds. Interestingly, direct application of PRP to culture media had no additive effects on osteogenesis of cells cultured on PRP coated PES/PVA scaffolds or those receiving typical osteogenic factors. The highest osteogenic effects were achieved by the simplest and most cost-effective method, i.e. merely by using PRP coated scaffolds. PRP coated PES/PVA scaffolds can maximally induce osteogenesis with no need for extrinsic factors. The major contribution of this paper to the current researches on bone regeneration is to suggest an easy, cost-effective approach to enhance osteogenesis via PRP coated scaffolds, with no additional external growth factors.
Similar content being viewed by others
References
Ahmadyan S, Kabiri M (2017) Osmolyte type and the osmolarity level affect chondrogenesis of mesenchymal stem cells. Appl Biochem Biotech 185:507–523. https://doi.org/10.1007/s12010-017-2647-5
Ahvaz HH, Mobasheri H, Bakhshandeh B, Shakhssalim N, Naji M, Dodel M, Soleimani M (2013) Mechanical characteristics of electrospun aligned PCL/PLLA nanofibrous scaffolds conduct cell differentiation in human bladder. Tissue Eng. https://doi.org/10.1166/jnn.2013.7193
Amiri B, Ghollasi M, Shahrousvand M, Kamali M, Salimi A (2016) Osteoblast differentiation of mesenchymal stem cells on modi fi ed PES-PEG electrospun fi brous composites loaded with Zn 2 SiO 4 bioceramic nanoparticles. Differentiation 1–11. https://doi.org/10.1016/j.diff.2016.08.001
Anitua E, Tejero R (2013) Platelet-rich plasma to improve the bio-functionality of biomaterials. BioDrugs. https://doi.org/10.1007/s40259-012-0004-3
Ardeshirylajimi A, Dinarvand P, Seyedjafari E, Langroudi L, Jamshidi Adegani F, Soleimani M (2013) Enhanced reconstruction of rat calvarial defects achieved by plasma-treated electrospun scaffolds and induced pluripotent stem cells. Cell Tissue Res 354:849–860. https://doi.org/10.1007/s00441-013-1693-8
Ardeshirylajimi A, Farhadian S, Adegani FJ, Mirzaei S, Zomorrod MS, Langroudi L, Doostmohammadi A, Seyedjafari E, Soleimani M (2015) Enhanced osteoconductivity of polyethersulphone nanofibres loaded with bioactive glass nanoparticles in in vitro and in vivo models. Cell Prolif 48:455–464. https://doi.org/10.1111/cpr.12198
Babaeijandaghi F, Shabani I, Seyedjafari E, Naraghi ZS, Vasei M, Haddadi-Asl V, Hesari KK, Soleimani M (2010) Accelerated epidermal regeneration and improved dermal reconstruction achieved by polyethersulfone nanofibers. Tissue Eng Part A 16:3527–3536. https://doi.org/10.1089/ten.tea.2009.0829
Cho HS, Song IH, Park S, Sung MC, Ahn M, Song KE (2011) KJLM individual variation in growth factor concentrations in platelet-rich plasma and its influence on human mesenchymal. Stem Cells. https://doi.org/10.3343/kjlm.2011.31.3.212
Díaz-gómez L, Alvarez-lorenzo C, Concheiro A, Silva M, Sheikh FA, Cantu T, Desai R, Garcia VL (2015) NIH public access 180–188. https://doi.org/10.1016/j.msec.2014.03.065.Biodegradable
Dodel M, Nejad NH, Bahrami SH, Soleimani M (2016) Modifying the mechanical properties of silk nanofiber scaffold by knitted orientation for regenerative medicine applications. Cell Mol Biol 62:16–25. https://doi.org/10.14715/cmb/2016.62.10.3
Fernandes G, Yang S (2016) Application of platelet-rich plasma with stem cells in bone and periodontal tissue engineering. Bone Res. https://doi.org/10.1038/boneres.2016.36
Fibroblasts HD (2008) Proliferation-promoting effect of platelet-rich plasma on human adipose–derived stem cells and human dermal fibroblasts. Plast Reconstr Surg. https://doi.org/10.1097/PRS.0b013e3181882046
Freitag J, Bates D, Boyd R, Shah K, Barnard A, Huguenin L, Tenen A (2016) Mesenchymal stem cell therapy in the treatment of osteoarthritis: reparative pathways, safety and efficacy – a review. BMC Musculoskelet. Disord. https://doi.org/10.1186/s12891-016-1085-9
Gomide VS, Zonari A, Ocarino NM, Goes AM, Pereira MM (2012) In vitro and in vivo osteogenic potential of bioactive glass—PVA hybrid scaffolds colonized by mesenchymal stem cells. Biomed Mater. https://doi.org/10.1088/1748-6041/7/1/015004
Gritsch K, Salles V, Attik GN (2014) Surface entrapment of fibronectin on electrospun PLGA scaffolds for periodontal. Tissue Eng 3:117–127. https://doi.org/10.1089/biores.2014.0015
Haddadi-asl V (2010) Prepared by electrospinning ch archive 19, pp 457–468
Hanaee H, Masoud A, Hamid S (2012) Effective combination of hydrostatic pressure and aligned nanofibrous scaffolds on human bladder smooth muscle cells: implication for bladder tissue engineering 2281–2290. https://doi.org/10.1007/s10856-012-4688-1
Hsieh W, Liau J (2013) Cell culture and characterization of cross-linked poly (vinyl alcohol) -g-starch 3D scaffold for tissue engineering. Carbohydr Polym 98:574–580. https://doi.org/10.1016/j.carbpol.2013.06.020
Kabiri M, Oraee-yazdani S, Dodel M, Hanaee-ahvaz H, Soudi S, Seyedjafari E, Salehi M, Soleimani M, Cell S, Cell S, Neurosurgery F, Hospital ST, Beheshti S, Cell S (2015) Original article : Cytocompatibility of a conductive nanofibrous carbon nanotube/poly (l-lactic acid) composite. EXCLI J 14:851–860
Kang KS, Hong JM, Kang JA, Rhie J, Jeong YH, Cho D (2013) Regulation of osteogenic differentiation of human adipose-derived stem cells by controlling electromagnetic field conditions. Exp Mol Med 45:e6–e9. https://doi.org/10.1038/emm.2013.11
Kokai LE, Marra K, Rubin JP (2014) Adipose stem cells: biology and clinical applications for tissue repair and regeneration. Transl Res 163:399–408. https://doi.org/10.1016/j.trsl.2013.11.009
Liao H, Chen C (2014) Osteogenic potential: comparison between bone marrow and adipose-derived mesenchymal stem cells. World J Stem Cells 6:288–295. https://doi.org/10.4252/wjsc.v6.i3.288
Liu Y, Lim J, Teoh S (2012) Review : development of clinically relevant scaffolds for vascularised bone tissue engineering. Biotechnol Adv. https://doi.org/10.1016/j.biotechadv.2012.10.003
Maheshwari SU (2013) Electrospinning of polyvinylalcohol—polycaprolactone composite scaffolds for tissue engineering applications. Polym Bull. https://doi.org/10.1007/s00289-013-1002-4
Maheshwari SU, Kumar SV, Nagiah N, Uma TS (2013) Electrospinning of polyvinylalcohol-polycaprolactone composite scaffolds for tissue engineering applications. Polym Bull 70:2995–3010. https://doi.org/10.1007/s00289-013-1002-4
Manuscript A (2013) NIH public access 30, pp 546–554. https://doi.org/10.1016/j.tibtech.2012.07.005.Recent
Modesti M, Boaretti C (2014) Electrospun polyethersulfone nanofiber membranes. Encycl Membr. https://doi.org/10.1007/978-3-642-40872-4
Morcos MW, Al-Jallad H, Hamdy R (2015) Comprehensive review of adipose stem cells and their implication in distraction osteogenesis and bone regeneration. BioMed Res Int. https://doi.org/10.1155/2015/842975
Nyberg E, Holmes C, Witham T, Grayson WL (2015) Growth factor-eluting technologies for bone tissue engineering. Drug Deliv Transl Res. https://doi.org/10.1007/s13346-015-0233-3
Oryan A, Alidadi S, Moshiri A (2015) Platelet-rich plasma for bone healing and regeneration. Expert Opin Biol Ther. https://doi.org/10.1517/14712598.2016.1118458
Oryan A, Alidadi S, Moshiri A (2016) Platelet-rich plasma for bone healing and regeneration. Expert Opin Biol Ther. https://doi.org/10.1517/14712598.2016.1118458
Pakfar A, Irani S, Hanaee-ahvaz H (2016) Expressions of pathologic markers in PRP based chondrogenic differentiation of human adipose derived stem cells. Tissue Cell. https://doi.org/10.1016/j.tice.2016.11.001
Pournaqi F, Farahmand M, Ardeshirylajimi A (2016) Increasing biocompatibility of scaffold made of polyethersulfone (PES) through combining with polyaniline (PANI). J Paramed Sci 7:1–6
Qi YY, Tai ZX, Sun DF, Chen JT, Ma HB, Yan XB, Liu B, Xue QJ (2013) Fabrication and characterization of poly(vinyl alcohol)/graphene oxide nanofibrous biocomposite scaffolds. J Appl Polym Sci 127:1885–1894. https://doi.org/10.1002/app.37924
Ramezanifard R, Kabiri M, Ahvaz HH, Tech- SC (2017) Original article: effects of platelet rich plasma and chondrocyte co-culture on MSC chondrogenesis, hypertrophy and pathological responses. EXCLI J 16:1031–1045
Schofer MD, Roessler PP, Schaefer J, Theisen C, Schlimme S, Heverhagen JT, Voelker M, Dersch R, Agarwal S, Fuchs S, Paletta RJ (2011) Electrospun PLLA nanofiber scaffolds and their use in combination with BMP-2 for reconstruction of bone defects. PLoS ONE. https://doi.org/10.1371/journal.pone.0025462
Song W, Markel DC, Wang S, Shi T, Mao G, Ren W (2012) Electrospun polyvinyl alcohol–collagen–hydroxyapatite nanofibers: a biomimetic extracellular matrix for osteoblastic cells. Nanotechnology 23:115101. https://doi.org/10.1088/0957-4484/23/11/115101
Sun H, Zhang Y, Dou L, Song X, Gu X, Fu C (2016) Nanofiber design for human stem cell culture. Rev Adv Mater Sci 44:160–167
Tajima S, Tobita M, Orbay H, Hyakusoku H, Mizuno H (2015) Direct and indirect effects of a combination of adipose-derivedstem cells and platelet-rich plasma on bone regeneration. Tissue Eng Part A 21(5–6):895–905. https://doi.org/10.1089/ten.TEA.2014.0336
Tobita M, Tajima S, Mizuno H (2015) Adipose tissue-derived mesenchymal stem cells and platelet-rich plasma: stem cell transplantation methods that enhance stemness. Stem Cell Res Ther 6:215. https://doi.org/10.1186/s13287-015-0217-8
Unger RE, Peters K, Huang Q, Funk A, Paul D, Kirkpatrick CJ (2005) Vascularization and gene regulation of human endothelial cells growing on porous polyethersulfone (PES) hollow fiber membranes. Biomaterials 26:3461–3469. https://doi.org/10.1016/j.biomaterials.2004.09.047
Yamaguchi DT (2014) “Ins” and “Outs” of mesenchymal stem cell osteogenesis in regenerative medicine. World J Stem Cells 6:94–110. https://doi.org/10.4252/wjsc.v6.i2.94
Zhang N, Wu Y, Qian S, Teng C, Chen S, Li H (2013) Research progress in the mechanism of effect of PRP in bone deficiency healing. Sci World J. https://doi.org/10.1155/2013/134582
Acknowledgements
This work was supported financially by Stem Cell Technology Research Center.
Author information
Authors and Affiliations
Corresponding authors
Ethics declarations
Conflict of interest
The authors declare no conflict of interest.
Rights and permissions
About this article
Cite this article
Kazem-Arki, M., Kabiri, M., Rad, I. et al. Enhancement of osteogenic differentiation of adipose-derived stem cells by PRP modified nanofibrous scaffold. Cytotechnology 70, 1487–1498 (2018). https://doi.org/10.1007/s10616-018-0226-4
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10616-018-0226-4