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Cytotechnology

, Volume 70, Issue 4, pp 1193–1204 | Cite as

Adipose tissue extract shows potential for wound healing: in vitro proliferation and migration of cell types contributing to wound healing in the presence of adipose tissue preparation and platelet rich plasma

  • Jenny F. LópezEmail author
  • Jertta-Riina Sarkanen
  • Outi Huttala
  • Ilkka S. Kaartinen
  • Hannu O. Kuokkanen
  • Timo Ylikomi
Article

Abstract

Growth factors are the key elements in wound healing signaling for cell migration, differentiation and proliferation. Platelet-rich plasma (PRP), one of the most studied sources of growth factors, has demonstrated to promote wound healing in vitro and in vivo. Adipose tissue is an alternative source of growth factors. Through a simple lipoaspirate method, adipose derived growth factor-rich preparation (adipose tissue extract; ATE) can be obtained. The authors set out to compare the effects of these two growth factor sources in cell proliferation and migration (scratch) assays of keratinocyte, fibroblast, endothelial and adipose derived stem cells. Growth factors involved in wound healing were measured: keratinocyte growth factor, epidermal growth factor, insulin-like growth factor, interleukin 6, platelet-derived growth factor beta, tumor necrosis factor alfa, transforming growth factor beta and vascular endothelial growth factor. PRP showed higher growth factor concentrations, except for keratinocyte growth factor, that was present in adipose tissue in greater quantities. This was reflected in vitro, where ATE significantly induced proliferation of keratinocytes at day 6 (p < 0.001), compared to plasma and control. Similarly, ATE-treated fibroblast and adipose stem cell cultures showed accelerated migration in scratch assays. Moreover, both sources showed accelerated keratinocyte migration. Adipose tissue preparation has an inductive effect in wound healing by proliferation and migration of cells involved in wound closure. Adipose tissue preparation appears to offer the distinct advantage of containing the adequate quantities of growth factors that induce cell activation, proliferation and migration, particularly in the early phase of wound healing.

Keywords

Platelet-rich plasma Adipose tissue extract Growth factors Cell proliferation Biomedical engineering 

Abbreviations

Ang-1

Angiopoietin 1

Ang-2

Angiopoietin 2

ATE

Adipose tissue extract

ECM

Extracellular matrix

EGF

Epidermal growth factor

bFGF

Basic fibroblast growth factor

IFN-γ

Interferon-gamma

IGF-1

Insulin-like growth factor

IL-6

Interleukin 6

HGF

Hepatocyte growth factor

KGF (FGF-7)

Keratinocyte growth factor (fibroblast growth factor 7)

PDGF-B

Platelet-derived growth factor beta

PRP

Platelet-rich plasma

TGF-α

Transforming growth factor alfa

TGF-β

Transforming growth factor beta

TNFα

Tumor necrosis factor alfa

TSBP

Thrombospondins

VEGF

Vascular endothelial growth factor

Notes

Acknowledgements

We would like to thank Ms. Hilkka Mäkinen for her invaluable work with laboratory assistance. Our thanks for volunteer patients and the health staff at the Tampere University Hospital.

Funding

This study was supported by the Finnish Technology and Innovation Agency TEKES, and Competitive State Research Financing of the Expert Responsibility area of Tampere University Hospital, Grant number 9S025.

Compliance with ethical standards

Conflict of interest

Patent issued in The USA (9056084 B2) and pending (WO2010026299A1) in other countries.

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • Jenny F. López
    • 1
    • 2
    Email author
  • Jertta-Riina Sarkanen
    • 1
    • 4
  • Outi Huttala
    • 3
  • Ilkka S. Kaartinen
    • 2
    • 4
  • Hannu O. Kuokkanen
    • 2
    • 4
  • Timo Ylikomi
    • 1
    • 4
  1. 1.Cell Biology, Faculty of Medicine and Life SciencesUniversity of TampereTampereFinland
  2. 2.Department of Plastic Surgery, Unit of Musculoskeletal DiseasesTampere University Hospital, Pirkanmaa Hospital DistrictTampereFinland
  3. 3.FICAM, Finnish Center for Alternative Methods, Faculty of Medicine and Life SciencesUniversity of TampereTampereFinland
  4. 4.Science CenterPirkanmaa Hospital DistrictTampereFinland

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