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Inhibitory effect of Japanese black vinegar on IgE-mediated degranulation of RBL-2H3 cells and a murine model of Japanese cedar pollinosis

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Abstract

Japanese black vinegar (JBV) is a traditional vinegar manufactured with steamed unpolished rice. After screening, beneficial effects of JBV on IgE-mediated allergic responses were found. In this study, acetic acid-free JBV was used to evaluate its antiallergic effects. JBV suppressed degranulation of rat basophilic leukemia RBL-2H3 cells in a dose-dependent manner without cytotoxicity. The inhibitory effect of JBV on the degranulation seemed to be caused by the bioactive ingredients other than proteins, because the activity was not affected by heat treatment or protease digestion. JBV inhibited the elevation in the intracellular Ca2+ concentration induced by antigen. Immunoblot analysis revealed that JBV suppresses degranulation of RBL-2H3 cells by downregulated phosphorylation of PI3K, Akt, and PLCγ1. In addition, oral administration of JBV significantly suppressed passive cutaneous anaphylaxis reaction in mice and an allergic symptom in Cry j1-induced pollinosis model mice. Thus, JBV has a potential as a health-promoting food with the antiallergy effect.

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Abbreviations

BSA:

Bovine serum albumin

[Ca2+]i :

Intracellular Ca2+ concentration

DMEM:

Dulbecco’s modified Eagle’s medium

DNP:

Dinitrophenol

ELISA:

Enzyme-linked immunosorbent assay

FBS:

Fetal bovine serum

FcεRI:

High-affinity IgE receptor

HSA:

Human serum albumin

IFN-γ:

Interferon-γ

IgE:

Immunoglobulin E

IL-4:

Interleukin-4

JBV:

Japanese black vinegar

NaPB:

Sodium phosphate buffer

PBS:

Phosphate-buffered saline

PCA:

Passive cutaneous anaphylaxis

PI3K:

Phosphoinositide 3-kinase

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Acknowledgements

Animal experiments were accomplished at the Division of Genetic Research of the Advanced Research Support Center (ADRES), Ehime University.

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Authors and Affiliations

  1. Graduate School of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime, 790-8566, Japan

    Seiji Awane, Kosuke Nishi, Momoko Ishida & Takuya Sugahara

  2. Food and Health Sciences Research Center, Ehime University, Matsuyama, Ehime, 790-8566, Japan

    Kosuke Nishi & Takuya Sugahara

  3. Sakamoto Kurozu Inc, 21-15 Uenosono-cho, Kagoshima, 890-0052, Japan

    Masanobu Nagano, Kazunori Hashiguchi & Akira Fujii

Authors
  1. Seiji Awane
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  2. Kosuke Nishi
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  3. Momoko Ishida
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  4. Masanobu Nagano
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  5. Kazunori Hashiguchi
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  6. Akira Fujii
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  7. Takuya Sugahara
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Corresponding author

Correspondence to Takuya Sugahara.

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Conflict of interest

Masanobu Nagano, Kazunori Hashiguchi, Akira Fujii, and Takuya Sugahara are inventors in a patent application filed by Ehime University, which disclose bioactive agents targeting mast cell degranulation described in the present article. The remaining authors declare no conflict of interest.

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Awane, S., Nishi, K., Ishida, M. et al. Inhibitory effect of Japanese black vinegar on IgE-mediated degranulation of RBL-2H3 cells and a murine model of Japanese cedar pollinosis. Cytotechnology 70, 961–974 (2018). https://doi.org/10.1007/s10616-018-0208-6

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  • DOI: https://doi.org/10.1007/s10616-018-0208-6

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